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Aquatic model for engine oil degradation by rhamnolipid producing Nocardiopsis VITSISB
The present study was focused on isolation, screening, characterization and application of biosurfactant producing marine actinobacteria. Twenty actinobacteria were isolated from marine water sample and were primarily screened for biosurfactant production using hemolytic activity method. Among the 2...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362736/ https://www.ncbi.nlm.nih.gov/pubmed/28324576 http://dx.doi.org/10.1007/s13205-014-0199-8 |
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author | Roy, Suki Chandni, Shreta Das, Ishita Karthik, Loganathan Kumar, Gaurav Bhaskara Rao, Kokati Venkata |
author_facet | Roy, Suki Chandni, Shreta Das, Ishita Karthik, Loganathan Kumar, Gaurav Bhaskara Rao, Kokati Venkata |
author_sort | Roy, Suki |
collection | PubMed |
description | The present study was focused on isolation, screening, characterization and application of biosurfactant producing marine actinobacteria. Twenty actinobacteria were isolated from marine water sample and were primarily screened for biosurfactant production using hemolytic activity method. Among the 20 isolates, six showed positive result for hemolytic activity and those were taken for further secondary screening tests such as oil collapse method, oil spreading method and emulsification method. From the results of secondary screening analysis, two isolates (SIS-3 and SIS-20) were selected and further used to carry out biosurfactant characterization test such as pH, density, surface tension and viscosity determination. Comparing biosurfactant characterization results, SIS-3 was chosen for further analysis and application. FT-IR and GC–MS were carried out for analysis of biosurfactant from isolate SIS-3 and the compound detected was rhamnolipid. The isolate (SIS-3) was identified as Nocardiopsis using 16S rRNA gene sequencing and named as ‘Nocardiopsis VITSISB’ (KC958579) which was further applied for immobilizing whole cells for engine oil degradation by constructing an aquatic model and using natural products such as soybean meal, sugarcane juice as nutrient source. The oil was efficiently degraded by rhamnolipid producing Nocardiopsis VITSISB (KC958579) within 25 days which indicated that the strain can act as a natural candidate for the bioremediation of oil spill in ocean. |
format | Online Article Text |
id | pubmed-4362736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-43627362015-03-24 Aquatic model for engine oil degradation by rhamnolipid producing Nocardiopsis VITSISB Roy, Suki Chandni, Shreta Das, Ishita Karthik, Loganathan Kumar, Gaurav Bhaskara Rao, Kokati Venkata 3 Biotech Original Article The present study was focused on isolation, screening, characterization and application of biosurfactant producing marine actinobacteria. Twenty actinobacteria were isolated from marine water sample and were primarily screened for biosurfactant production using hemolytic activity method. Among the 20 isolates, six showed positive result for hemolytic activity and those were taken for further secondary screening tests such as oil collapse method, oil spreading method and emulsification method. From the results of secondary screening analysis, two isolates (SIS-3 and SIS-20) were selected and further used to carry out biosurfactant characterization test such as pH, density, surface tension and viscosity determination. Comparing biosurfactant characterization results, SIS-3 was chosen for further analysis and application. FT-IR and GC–MS were carried out for analysis of biosurfactant from isolate SIS-3 and the compound detected was rhamnolipid. The isolate (SIS-3) was identified as Nocardiopsis using 16S rRNA gene sequencing and named as ‘Nocardiopsis VITSISB’ (KC958579) which was further applied for immobilizing whole cells for engine oil degradation by constructing an aquatic model and using natural products such as soybean meal, sugarcane juice as nutrient source. The oil was efficiently degraded by rhamnolipid producing Nocardiopsis VITSISB (KC958579) within 25 days which indicated that the strain can act as a natural candidate for the bioremediation of oil spill in ocean. Springer Berlin Heidelberg 2014-03-26 2015-04 /pmc/articles/PMC4362736/ /pubmed/28324576 http://dx.doi.org/10.1007/s13205-014-0199-8 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ This article is published under license to BioMed Central Ltd. Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Article Roy, Suki Chandni, Shreta Das, Ishita Karthik, Loganathan Kumar, Gaurav Bhaskara Rao, Kokati Venkata Aquatic model for engine oil degradation by rhamnolipid producing Nocardiopsis VITSISB |
title | Aquatic model for engine oil degradation by rhamnolipid producing Nocardiopsis VITSISB |
title_full | Aquatic model for engine oil degradation by rhamnolipid producing Nocardiopsis VITSISB |
title_fullStr | Aquatic model for engine oil degradation by rhamnolipid producing Nocardiopsis VITSISB |
title_full_unstemmed | Aquatic model for engine oil degradation by rhamnolipid producing Nocardiopsis VITSISB |
title_short | Aquatic model for engine oil degradation by rhamnolipid producing Nocardiopsis VITSISB |
title_sort | aquatic model for engine oil degradation by rhamnolipid producing nocardiopsis vitsisb |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362736/ https://www.ncbi.nlm.nih.gov/pubmed/28324576 http://dx.doi.org/10.1007/s13205-014-0199-8 |
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