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Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity

In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH(...

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Autores principales: Gowramma, B., Keerthi, U., Rafi, Mokula, Muralidhara Rao, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362738/
https://www.ncbi.nlm.nih.gov/pubmed/28324578
http://dx.doi.org/10.1007/s13205-014-0210-4
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author Gowramma, B.
Keerthi, U.
Rafi, Mokula
Muralidhara Rao, D.
author_facet Gowramma, B.
Keerthi, U.
Rafi, Mokula
Muralidhara Rao, D.
author_sort Gowramma, B.
collection PubMed
description In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH(3))(2)](+)) containing 1 mM AgNO(3). Synthesized silver nanoparticles (AgNPs) were characterized by ultraviolet–visible spectroscopy and energy-dispersive X-ray (EDX) spectroscopy analysis. Morphological study of silver nanoparticles was carried out using transmission electron microscopy (TEM) and scanning electron microscope (SEM). The spherical morphology of silver nanoparticles was confirmed from SEM image. The TEM image showed the average particle size of silver nanoparticles was about 15 nm. Silver nanoparticles synthesized from C. glutamicum were found to have enhanced antimicrobial activity against selected pathogenic strains. Silver nanoparticles from pure strains of Corynebacterium species was done by many investigators, but as per the present literature, this is the first report on the production of silver nanoparticles using a native strain of Corynebacterium.
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spelling pubmed-43627382015-03-24 Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity Gowramma, B. Keerthi, U. Rafi, Mokula Muralidhara Rao, D. 3 Biotech Original Article In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH(3))(2)](+)) containing 1 mM AgNO(3). Synthesized silver nanoparticles (AgNPs) were characterized by ultraviolet–visible spectroscopy and energy-dispersive X-ray (EDX) spectroscopy analysis. Morphological study of silver nanoparticles was carried out using transmission electron microscopy (TEM) and scanning electron microscope (SEM). The spherical morphology of silver nanoparticles was confirmed from SEM image. The TEM image showed the average particle size of silver nanoparticles was about 15 nm. Silver nanoparticles synthesized from C. glutamicum were found to have enhanced antimicrobial activity against selected pathogenic strains. Silver nanoparticles from pure strains of Corynebacterium species was done by many investigators, but as per the present literature, this is the first report on the production of silver nanoparticles using a native strain of Corynebacterium. Springer Berlin Heidelberg 2014-04-08 2015-04 /pmc/articles/PMC4362738/ /pubmed/28324578 http://dx.doi.org/10.1007/s13205-014-0210-4 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ This article is published under license to BioMed Central Ltd. Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Gowramma, B.
Keerthi, U.
Rafi, Mokula
Muralidhara Rao, D.
Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity
title Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity
title_full Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity
title_fullStr Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity
title_full_unstemmed Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity
title_short Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity
title_sort biogenic silver nanoparticles production and characterization from native stain of corynebacterium species and its antimicrobial activity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362738/
https://www.ncbi.nlm.nih.gov/pubmed/28324578
http://dx.doi.org/10.1007/s13205-014-0210-4
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