Cargando…

A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation

The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the orga...

Descripción completa

Detalles Bibliográficos
Autores principales: Prajapati, Vimal S., Trivedi, Ujjval B., Patel, Kamlesh C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362744/
https://www.ncbi.nlm.nih.gov/pubmed/28324580
http://dx.doi.org/10.1007/s13205-014-0213-1
_version_ 1782361839980511232
author Prajapati, Vimal S.
Trivedi, Ujjval B.
Patel, Kamlesh C.
author_facet Prajapati, Vimal S.
Trivedi, Ujjval B.
Patel, Kamlesh C.
author_sort Prajapati, Vimal S.
collection PubMed
description The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-state fermentation using wheat bran as the substrate. The medium components influencing the enzyme production were identified using a two-level fractional factorial Plackett–Burman design. Among the various variables screened, starch, ammonium sulphate and calcium chloride were found to be most significant medium components. The optimum levels of these significant parameters were determined employing the response surface Central Composite design which significantly increased the enzyme production with the supplementation of starch 0.01 g, ammonium sulphate 0.2 g and 5 mM calcium chloride in the production medium. Temperature and pH stability of the alpha-amylase suggested its wide application in the food and pharmaceutical industries.
format Online
Article
Text
id pubmed-4362744
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-43627442015-03-24 A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation Prajapati, Vimal S. Trivedi, Ujjval B. Patel, Kamlesh C. 3 Biotech Original Article The bacterial strain producing thermostable, alklophilic alpha-amylase was identified as Bacillus amyloliquefaciens KCP2 using 16S rDNA gene sequencing data (NCBI Accession No: KF112071). Medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-state fermentation using wheat bran as the substrate. The medium components influencing the enzyme production were identified using a two-level fractional factorial Plackett–Burman design. Among the various variables screened, starch, ammonium sulphate and calcium chloride were found to be most significant medium components. The optimum levels of these significant parameters were determined employing the response surface Central Composite design which significantly increased the enzyme production with the supplementation of starch 0.01 g, ammonium sulphate 0.2 g and 5 mM calcium chloride in the production medium. Temperature and pH stability of the alpha-amylase suggested its wide application in the food and pharmaceutical industries. Springer Berlin Heidelberg 2014-04-11 2015-04 /pmc/articles/PMC4362744/ /pubmed/28324580 http://dx.doi.org/10.1007/s13205-014-0213-1 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Prajapati, Vimal S.
Trivedi, Ujjval B.
Patel, Kamlesh C.
A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation
title A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation
title_full A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation
title_fullStr A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation
title_full_unstemmed A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation
title_short A statistical approach for the production of thermostable and alklophilic alpha-amylase from Bacillus amyloliquefaciens KCP2 under solid-state fermentation
title_sort statistical approach for the production of thermostable and alklophilic alpha-amylase from bacillus amyloliquefaciens kcp2 under solid-state fermentation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362744/
https://www.ncbi.nlm.nih.gov/pubmed/28324580
http://dx.doi.org/10.1007/s13205-014-0213-1
work_keys_str_mv AT prajapativimals astatisticalapproachfortheproductionofthermostableandalklophilicalphaamylasefrombacillusamyloliquefacienskcp2undersolidstatefermentation
AT trivediujjvalb astatisticalapproachfortheproductionofthermostableandalklophilicalphaamylasefrombacillusamyloliquefacienskcp2undersolidstatefermentation
AT patelkamleshc astatisticalapproachfortheproductionofthermostableandalklophilicalphaamylasefrombacillusamyloliquefacienskcp2undersolidstatefermentation
AT prajapativimals statisticalapproachfortheproductionofthermostableandalklophilicalphaamylasefrombacillusamyloliquefacienskcp2undersolidstatefermentation
AT trivediujjvalb statisticalapproachfortheproductionofthermostableandalklophilicalphaamylasefrombacillusamyloliquefacienskcp2undersolidstatefermentation
AT patelkamleshc statisticalapproachfortheproductionofthermostableandalklophilicalphaamylasefrombacillusamyloliquefacienskcp2undersolidstatefermentation