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Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley
The microbial communities inhabiting the root interior of healthy plants, as well as the rhizosphere, which consists of soil particles firmly attached to roots, engage in symbiotic associations with their host. To investigate the structural and functional diversification among these communities, we...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362959/ https://www.ncbi.nlm.nih.gov/pubmed/25732064 http://dx.doi.org/10.1016/j.chom.2015.01.011 |
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author | Bulgarelli, Davide Garrido-Oter, Ruben Münch, Philipp C. Weiman, Aaron Dröge, Johannes Pan, Yao McHardy, Alice C. Schulze-Lefert, Paul |
author_facet | Bulgarelli, Davide Garrido-Oter, Ruben Münch, Philipp C. Weiman, Aaron Dröge, Johannes Pan, Yao McHardy, Alice C. Schulze-Lefert, Paul |
author_sort | Bulgarelli, Davide |
collection | PubMed |
description | The microbial communities inhabiting the root interior of healthy plants, as well as the rhizosphere, which consists of soil particles firmly attached to roots, engage in symbiotic associations with their host. To investigate the structural and functional diversification among these communities, we employed a combination of 16S rRNA gene profiling and shotgun metagenome analysis of the microbiota associated with wild and domesticated accessions of barley (Hordeum vulgare). Bacterial families Comamonadaceae, Flavobacteriaceae, and Rhizobiaceae dominate the barley root-enriched microbiota. Host genotype has a small, but significant, effect on the diversity of root-associated bacterial communities, possibly representing a footprint of barley domestication. Traits related to pathogenesis, secretion, phage interactions, and nutrient mobilization are enriched in the barley root-associated microbiota. Strikingly, protein families assigned to these same traits showed evidence of positive selection. Our results indicate that the combined action of microbe-microbe and host-microbe interactions drives microbiota differentiation at the root-soil interface. |
format | Online Article Text |
id | pubmed-4362959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43629592015-04-01 Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley Bulgarelli, Davide Garrido-Oter, Ruben Münch, Philipp C. Weiman, Aaron Dröge, Johannes Pan, Yao McHardy, Alice C. Schulze-Lefert, Paul Cell Host Microbe Resource The microbial communities inhabiting the root interior of healthy plants, as well as the rhizosphere, which consists of soil particles firmly attached to roots, engage in symbiotic associations with their host. To investigate the structural and functional diversification among these communities, we employed a combination of 16S rRNA gene profiling and shotgun metagenome analysis of the microbiota associated with wild and domesticated accessions of barley (Hordeum vulgare). Bacterial families Comamonadaceae, Flavobacteriaceae, and Rhizobiaceae dominate the barley root-enriched microbiota. Host genotype has a small, but significant, effect on the diversity of root-associated bacterial communities, possibly representing a footprint of barley domestication. Traits related to pathogenesis, secretion, phage interactions, and nutrient mobilization are enriched in the barley root-associated microbiota. Strikingly, protein families assigned to these same traits showed evidence of positive selection. Our results indicate that the combined action of microbe-microbe and host-microbe interactions drives microbiota differentiation at the root-soil interface. Cell Press 2015-03-11 /pmc/articles/PMC4362959/ /pubmed/25732064 http://dx.doi.org/10.1016/j.chom.2015.01.011 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Resource Bulgarelli, Davide Garrido-Oter, Ruben Münch, Philipp C. Weiman, Aaron Dröge, Johannes Pan, Yao McHardy, Alice C. Schulze-Lefert, Paul Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley |
title | Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley |
title_full | Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley |
title_fullStr | Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley |
title_full_unstemmed | Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley |
title_short | Structure and Function of the Bacterial Root Microbiota in Wild and Domesticated Barley |
title_sort | structure and function of the bacterial root microbiota in wild and domesticated barley |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362959/ https://www.ncbi.nlm.nih.gov/pubmed/25732064 http://dx.doi.org/10.1016/j.chom.2015.01.011 |
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