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Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-A(y)

The A(y) allele at the agouti locus causes obesity and promotes linear growth in mice. However, body weight gain stops between 16 and 17 weeks after birth, and then, body weight decreases gradually in DDD.Cg-A(y) male mice. Body weight loss is a consequence of diabetes mellitus, which is genetically...

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Autores principales: SUTO, Jun-ichi, SATOU, Kunio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363023/
https://www.ncbi.nlm.nih.gov/pubmed/25373882
http://dx.doi.org/10.1292/jvms.14-0351
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author SUTO, Jun-ichi
SATOU, Kunio
author_facet SUTO, Jun-ichi
SATOU, Kunio
author_sort SUTO, Jun-ichi
collection PubMed
description The A(y) allele at the agouti locus causes obesity and promotes linear growth in mice. However, body weight gain stops between 16 and 17 weeks after birth, and then, body weight decreases gradually in DDD.Cg-A(y) male mice. Body weight loss is a consequence of diabetes mellitus, which is genetically controlled mainly by a quantitative trait locus (QTL) on chromosome 4. This study aimed to further characterize diabetes mellitus and body weight loss in DDD.Cg-A(y) males. The number of β-cells was markedly reduced, and plasma insulin levels were very low in the DDD.Cg-A(y) males. Using a backcross progeny of DDD × (B6 × DDD.Cg-A(y)) F(1)-A(y), we identified one significant QTL for plasma insulin levels on distal chromosome 4, which was coincidental with QTL for hyperglycemia and lower body weight. The DDD allele was associated with decreased plasma insulin levels. When the DDD.Cg-A(y) males were housed under three different housing conditions [group housing (4 or 5 DDD.Cg-A(y) and DDD males), individual housing (single DDD.Cg-A(y) male) and single male housing with females (single DDD.Cg-A(y) male with DDD.Cg-A(y) or DDD females)], diabetes mellitus and body weight loss were most severely expressed in individually housed mice. Thus, the severity of diabetes and body weight loss in the DDD.Cg-A(y) males was strongly influenced by the housing conditions. These results demonstrate that both genetic and nongenetic environmental factors are involved in the development of diabetes mellitus and body weight loss in the DDD.Cg-A(y) males.
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spelling pubmed-43630232015-03-19 Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-A(y) SUTO, Jun-ichi SATOU, Kunio J Vet Med Sci Laboratory Animal Science The A(y) allele at the agouti locus causes obesity and promotes linear growth in mice. However, body weight gain stops between 16 and 17 weeks after birth, and then, body weight decreases gradually in DDD.Cg-A(y) male mice. Body weight loss is a consequence of diabetes mellitus, which is genetically controlled mainly by a quantitative trait locus (QTL) on chromosome 4. This study aimed to further characterize diabetes mellitus and body weight loss in DDD.Cg-A(y) males. The number of β-cells was markedly reduced, and plasma insulin levels were very low in the DDD.Cg-A(y) males. Using a backcross progeny of DDD × (B6 × DDD.Cg-A(y)) F(1)-A(y), we identified one significant QTL for plasma insulin levels on distal chromosome 4, which was coincidental with QTL for hyperglycemia and lower body weight. The DDD allele was associated with decreased plasma insulin levels. When the DDD.Cg-A(y) males were housed under three different housing conditions [group housing (4 or 5 DDD.Cg-A(y) and DDD males), individual housing (single DDD.Cg-A(y) male) and single male housing with females (single DDD.Cg-A(y) male with DDD.Cg-A(y) or DDD females)], diabetes mellitus and body weight loss were most severely expressed in individually housed mice. Thus, the severity of diabetes and body weight loss in the DDD.Cg-A(y) males was strongly influenced by the housing conditions. These results demonstrate that both genetic and nongenetic environmental factors are involved in the development of diabetes mellitus and body weight loss in the DDD.Cg-A(y) males. The Japanese Society of Veterinary Science 2014-11-06 2015-02 /pmc/articles/PMC4363023/ /pubmed/25373882 http://dx.doi.org/10.1292/jvms.14-0351 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Laboratory Animal Science
SUTO, Jun-ichi
SATOU, Kunio
Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-A(y)
title Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-A(y)
title_full Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-A(y)
title_fullStr Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-A(y)
title_full_unstemmed Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-A(y)
title_short Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-A(y)
title_sort further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain ddd.cg-a(y)
topic Laboratory Animal Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363023/
https://www.ncbi.nlm.nih.gov/pubmed/25373882
http://dx.doi.org/10.1292/jvms.14-0351
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