Detection of avian-like rotavirus A VP4 from a calf in Japan

A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011–2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares...

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Autores principales: MITAKE, Hiromichi, ITO, Naoto, OKADERA, Kota, OKADA, Kazuma, NAKAGAWA, Kento, TANAKA, Tomomi, KATSURAGI, Kiyohito, KASAHARA, Kasumi, NIHONGI, Toshihide, TSUNEMITSU, Hiroshi, SUGIYAMA, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363026/
https://www.ncbi.nlm.nih.gov/pubmed/25311984
http://dx.doi.org/10.1292/jvms.14-0379
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author MITAKE, Hiromichi
ITO, Naoto
OKADERA, Kota
OKADA, Kazuma
NAKAGAWA, Kento
TANAKA, Tomomi
KATSURAGI, Kiyohito
KASAHARA, Kasumi
NIHONGI, Toshihide
TSUNEMITSU, Hiroshi
SUGIYAMA, Makoto
author_facet MITAKE, Hiromichi
ITO, Naoto
OKADERA, Kota
OKADA, Kazuma
NAKAGAWA, Kento
TANAKA, Tomomi
KATSURAGI, Kiyohito
KASAHARA, Kasumi
NIHONGI, Toshihide
TSUNEMITSU, Hiroshi
SUGIYAMA, Makoto
author_sort MITAKE, Hiromichi
collection PubMed
description A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011–2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. Phylogenetic analysis also revealed a close genetic relationship between N2342 and avian RVAs, suggesting bird-to-cattle transmission. We observed frequent contact of wild birds with calves in the farm, suggesting that these birds were the source of the virus.
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spelling pubmed-43630262015-03-19 Detection of avian-like rotavirus A VP4 from a calf in Japan MITAKE, Hiromichi ITO, Naoto OKADERA, Kota OKADA, Kazuma NAKAGAWA, Kento TANAKA, Tomomi KATSURAGI, Kiyohito KASAHARA, Kasumi NIHONGI, Toshihide TSUNEMITSU, Hiroshi SUGIYAMA, Makoto J Vet Med Sci Virology A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011–2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. Phylogenetic analysis also revealed a close genetic relationship between N2342 and avian RVAs, suggesting bird-to-cattle transmission. We observed frequent contact of wild birds with calves in the farm, suggesting that these birds were the source of the virus. The Japanese Society of Veterinary Science 2014-10-13 2015-02 /pmc/articles/PMC4363026/ /pubmed/25311984 http://dx.doi.org/10.1292/jvms.14-0379 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
MITAKE, Hiromichi
ITO, Naoto
OKADERA, Kota
OKADA, Kazuma
NAKAGAWA, Kento
TANAKA, Tomomi
KATSURAGI, Kiyohito
KASAHARA, Kasumi
NIHONGI, Toshihide
TSUNEMITSU, Hiroshi
SUGIYAMA, Makoto
Detection of avian-like rotavirus A VP4 from a calf in Japan
title Detection of avian-like rotavirus A VP4 from a calf in Japan
title_full Detection of avian-like rotavirus A VP4 from a calf in Japan
title_fullStr Detection of avian-like rotavirus A VP4 from a calf in Japan
title_full_unstemmed Detection of avian-like rotavirus A VP4 from a calf in Japan
title_short Detection of avian-like rotavirus A VP4 from a calf in Japan
title_sort detection of avian-like rotavirus a vp4 from a calf in japan
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363026/
https://www.ncbi.nlm.nih.gov/pubmed/25311984
http://dx.doi.org/10.1292/jvms.14-0379
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