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Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats

The peach tree, Prunus persica (L.) Batsch, is widely cultivated in China, and its flowers have been used for centuries in traditional Chinese medicine to treat gut motility disorders. But few studies have explored the pharmacological effect of Prunus persica (L.) Batsch flowers on gastrointestinal...

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Autores principales: Han, Wei, Xu, Jing Dong, Wei, Feng Xian, Zheng, Yong Dong, Ma, Jian Zhong, Xu, Xiao Dong, Wei, Zhen Gang, Wang, Wen, Zhang, You Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363542/
https://www.ncbi.nlm.nih.gov/pubmed/25821812
http://dx.doi.org/10.1155/2015/569853
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author Han, Wei
Xu, Jing Dong
Wei, Feng Xian
Zheng, Yong Dong
Ma, Jian Zhong
Xu, Xiao Dong
Wei, Zhen Gang
Wang, Wen
Zhang, You Cheng
author_facet Han, Wei
Xu, Jing Dong
Wei, Feng Xian
Zheng, Yong Dong
Ma, Jian Zhong
Xu, Xiao Dong
Wei, Zhen Gang
Wang, Wen
Zhang, You Cheng
author_sort Han, Wei
collection PubMed
description The peach tree, Prunus persica (L.) Batsch, is widely cultivated in China, and its flowers have been used for centuries in traditional Chinese medicine to treat gut motility disorders. But few studies have explored the pharmacological effect of Prunus persica (L.) Batsch flowers on gastrointestinal motility. In this study, the activities of different extracts from Prunus persica (L.) Batsch flowers on the smooth muscle contractions were evaluated using isolated colon model, and the ethyl acetate extract (EAE) showed the strongest effects in vitro. EAE (10(−8)–10(−5) g/mL) caused a concentration-dependent stimulatory effect in rat colonic tissue. Additionally, ketotifen (100 µM), cimetidine (10 µM), and pyrilamine (1 µM) produced a significant inhibition of contractions caused by EAE. Furthermore, immunofluorescence and toluidine blue staining revealed increased numbers of mast cells in the EAE group, and EAE increased histamine release from the colonic tissues. These data indicate that EAE has significant prokinetic activity and acts by a mechanism that mainly involves mast cell degranulation. Our study provides a pharmacological basis for the use of an extract of Prunus persica (L.) Batsch flowers in the treatment of gut motility disorders.
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spelling pubmed-43635422015-03-29 Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats Han, Wei Xu, Jing Dong Wei, Feng Xian Zheng, Yong Dong Ma, Jian Zhong Xu, Xiao Dong Wei, Zhen Gang Wang, Wen Zhang, You Cheng Biomed Res Int Research Article The peach tree, Prunus persica (L.) Batsch, is widely cultivated in China, and its flowers have been used for centuries in traditional Chinese medicine to treat gut motility disorders. But few studies have explored the pharmacological effect of Prunus persica (L.) Batsch flowers on gastrointestinal motility. In this study, the activities of different extracts from Prunus persica (L.) Batsch flowers on the smooth muscle contractions were evaluated using isolated colon model, and the ethyl acetate extract (EAE) showed the strongest effects in vitro. EAE (10(−8)–10(−5) g/mL) caused a concentration-dependent stimulatory effect in rat colonic tissue. Additionally, ketotifen (100 µM), cimetidine (10 µM), and pyrilamine (1 µM) produced a significant inhibition of contractions caused by EAE. Furthermore, immunofluorescence and toluidine blue staining revealed increased numbers of mast cells in the EAE group, and EAE increased histamine release from the colonic tissues. These data indicate that EAE has significant prokinetic activity and acts by a mechanism that mainly involves mast cell degranulation. Our study provides a pharmacological basis for the use of an extract of Prunus persica (L.) Batsch flowers in the treatment of gut motility disorders. Hindawi Publishing Corporation 2015 2015-03-02 /pmc/articles/PMC4363542/ /pubmed/25821812 http://dx.doi.org/10.1155/2015/569853 Text en Copyright © 2015 Wei Han et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Han, Wei
Xu, Jing Dong
Wei, Feng Xian
Zheng, Yong Dong
Ma, Jian Zhong
Xu, Xiao Dong
Wei, Zhen Gang
Wang, Wen
Zhang, You Cheng
Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats
title Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats
title_full Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats
title_fullStr Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats
title_full_unstemmed Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats
title_short Prokinetic Activity of Prunus persica (L.) Batsch Flowers Extract and Its Possible Mechanism of Action in Rats
title_sort prokinetic activity of prunus persica (l.) batsch flowers extract and its possible mechanism of action in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363542/
https://www.ncbi.nlm.nih.gov/pubmed/25821812
http://dx.doi.org/10.1155/2015/569853
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