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Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model
Topical administration of powdered shells of the land snail Megalobulimus lopesi was evaluated in Wistar rats for their healing activity in an excision wound model. The animals were distributed into three groups—G1 (control): no therapeutic intervention; G2 (vehicle controls): Lanette cream once dai...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363580/ https://www.ncbi.nlm.nih.gov/pubmed/25821475 http://dx.doi.org/10.1155/2015/120785 |
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author | Andrade, Paulo Henrique Muleta Schmidt Rondon, Eric Carollo, Carlos Alexandre Rodrigues Macedo, Maria Lígia Viana, Luiz Henrique Schiaveto de Souza, Albert Turatti Oliveira, Carolina Cepa Matos, Maria de Fatima |
author_facet | Andrade, Paulo Henrique Muleta Schmidt Rondon, Eric Carollo, Carlos Alexandre Rodrigues Macedo, Maria Lígia Viana, Luiz Henrique Schiaveto de Souza, Albert Turatti Oliveira, Carolina Cepa Matos, Maria de Fatima |
author_sort | Andrade, Paulo Henrique Muleta |
collection | PubMed |
description | Topical administration of powdered shells of the land snail Megalobulimus lopesi was evaluated in Wistar rats for their healing activity in an excision wound model. The animals were distributed into three groups—G1 (control): no therapeutic intervention; G2 (vehicle controls): Lanette cream once daily; G3 (experimental animals): treated with powdered shells. Variables investigated were: wound area contraction, angiogenic activity, morphometric data, leukocytic inflammatory infiltrate, and total leukocyte count in peripheral blood. Thermogravimetric analysis and quantification and characterization of powdered shell proteins were also performed. Wound area on days 3, 7, and 14 was smaller in G3, besides presenting wound closure on day 21 for all these animals. Topical administration of the powdered shells also led to an increased number of vessels at the wound site, higher leukocyte counts in peripheral blood, and increased leukocytic inflammatory infiltrate. The results lend support to the southern Brazilian folk use of M. lopesi powdered shells, as shown by the enhanced secondary-intention healing achieved with their topical administration to wounds in rats. Topical administration caused inflammatory response modulation, crucial to accelerating the healing process, the chronification of which increases the risks of wound contamination by opportunistic pathogens. |
format | Online Article Text |
id | pubmed-4363580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43635802015-03-29 Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model Andrade, Paulo Henrique Muleta Schmidt Rondon, Eric Carollo, Carlos Alexandre Rodrigues Macedo, Maria Lígia Viana, Luiz Henrique Schiaveto de Souza, Albert Turatti Oliveira, Carolina Cepa Matos, Maria de Fatima Evid Based Complement Alternat Med Research Article Topical administration of powdered shells of the land snail Megalobulimus lopesi was evaluated in Wistar rats for their healing activity in an excision wound model. The animals were distributed into three groups—G1 (control): no therapeutic intervention; G2 (vehicle controls): Lanette cream once daily; G3 (experimental animals): treated with powdered shells. Variables investigated were: wound area contraction, angiogenic activity, morphometric data, leukocytic inflammatory infiltrate, and total leukocyte count in peripheral blood. Thermogravimetric analysis and quantification and characterization of powdered shell proteins were also performed. Wound area on days 3, 7, and 14 was smaller in G3, besides presenting wound closure on day 21 for all these animals. Topical administration of the powdered shells also led to an increased number of vessels at the wound site, higher leukocyte counts in peripheral blood, and increased leukocytic inflammatory infiltrate. The results lend support to the southern Brazilian folk use of M. lopesi powdered shells, as shown by the enhanced secondary-intention healing achieved with their topical administration to wounds in rats. Topical administration caused inflammatory response modulation, crucial to accelerating the healing process, the chronification of which increases the risks of wound contamination by opportunistic pathogens. Hindawi Publishing Corporation 2015 2015-03-02 /pmc/articles/PMC4363580/ /pubmed/25821475 http://dx.doi.org/10.1155/2015/120785 Text en Copyright © 2015 Paulo Henrique Muleta Andrade et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Andrade, Paulo Henrique Muleta Schmidt Rondon, Eric Carollo, Carlos Alexandre Rodrigues Macedo, Maria Lígia Viana, Luiz Henrique Schiaveto de Souza, Albert Turatti Oliveira, Carolina Cepa Matos, Maria de Fatima Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model |
title | Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model |
title_full | Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model |
title_fullStr | Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model |
title_full_unstemmed | Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model |
title_short | Effect of Powdered Shells of the Snail Megalobulimus lopesi on Secondary-Intention Wound Healing in an Animal Model |
title_sort | effect of powdered shells of the snail megalobulimus lopesi on secondary-intention wound healing in an animal model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363580/ https://www.ncbi.nlm.nih.gov/pubmed/25821475 http://dx.doi.org/10.1155/2015/120785 |
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