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Detection of BRAF mutation in Chinese tumor patients using a highly sensitive antibody immunohistochemistry assay
BRAF mutations can be found in various solid tumors. But accurate and reliable screening for BRAF mutation that is compatible for clinical application is not yet available. In this study, we used an automated immunohistochemistry (IHC) staining coupled with mouse monoclonal anti-BRAF V600E (VE1) pri...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363828/ https://www.ncbi.nlm.nih.gov/pubmed/25784606 http://dx.doi.org/10.1038/srep09211 |
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author | Qiu, Tian Lu, Haizhen Guo, Lei Huang, Wenting Ling, Yun Shan, Ling Li, Wenbin Ying, Jianming Lv, Ning |
author_facet | Qiu, Tian Lu, Haizhen Guo, Lei Huang, Wenting Ling, Yun Shan, Ling Li, Wenbin Ying, Jianming Lv, Ning |
author_sort | Qiu, Tian |
collection | PubMed |
description | BRAF mutations can be found in various solid tumors. But accurate and reliable screening for BRAF mutation that is compatible for clinical application is not yet available. In this study, we used an automated immunohistochemistry (IHC) staining coupled with mouse monoclonal anti-BRAF V600E (VE1) primary antibody to screen the BRAF V600E mutation in 779 tumor cases, including 611 colorectal carcinomas (CRC), 127 papillary thyroid carcinomas (PTC) and 41 malignant melanomas. Among the 779 cases, 150 cases were positive for BRAF (V600E) staining, including 38 (of 611, 6%) CRCs, 102 (of 127, 80%) PTCs and 10 (of 41, 24%) malignant melanomas. Sanger sequencing and real-time PCR confirmed the sensitivity and specificity of IHC staining for the V600E mutation are 100% and 99%, respectively. Therefore, our study demonstrates that the fully automated IHC is a reliable tool to determine BRAF mutation status in CRC, PTC and melanoma and can be used for routine clinical screen. |
format | Online Article Text |
id | pubmed-4363828 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43638282015-03-27 Detection of BRAF mutation in Chinese tumor patients using a highly sensitive antibody immunohistochemistry assay Qiu, Tian Lu, Haizhen Guo, Lei Huang, Wenting Ling, Yun Shan, Ling Li, Wenbin Ying, Jianming Lv, Ning Sci Rep Article BRAF mutations can be found in various solid tumors. But accurate and reliable screening for BRAF mutation that is compatible for clinical application is not yet available. In this study, we used an automated immunohistochemistry (IHC) staining coupled with mouse monoclonal anti-BRAF V600E (VE1) primary antibody to screen the BRAF V600E mutation in 779 tumor cases, including 611 colorectal carcinomas (CRC), 127 papillary thyroid carcinomas (PTC) and 41 malignant melanomas. Among the 779 cases, 150 cases were positive for BRAF (V600E) staining, including 38 (of 611, 6%) CRCs, 102 (of 127, 80%) PTCs and 10 (of 41, 24%) malignant melanomas. Sanger sequencing and real-time PCR confirmed the sensitivity and specificity of IHC staining for the V600E mutation are 100% and 99%, respectively. Therefore, our study demonstrates that the fully automated IHC is a reliable tool to determine BRAF mutation status in CRC, PTC and melanoma and can be used for routine clinical screen. Nature Publishing Group 2015-03-18 /pmc/articles/PMC4363828/ /pubmed/25784606 http://dx.doi.org/10.1038/srep09211 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Qiu, Tian Lu, Haizhen Guo, Lei Huang, Wenting Ling, Yun Shan, Ling Li, Wenbin Ying, Jianming Lv, Ning Detection of BRAF mutation in Chinese tumor patients using a highly sensitive antibody immunohistochemistry assay |
title | Detection of BRAF mutation in Chinese tumor patients using a highly sensitive antibody immunohistochemistry assay |
title_full | Detection of BRAF mutation in Chinese tumor patients using a highly sensitive antibody immunohistochemistry assay |
title_fullStr | Detection of BRAF mutation in Chinese tumor patients using a highly sensitive antibody immunohistochemistry assay |
title_full_unstemmed | Detection of BRAF mutation in Chinese tumor patients using a highly sensitive antibody immunohistochemistry assay |
title_short | Detection of BRAF mutation in Chinese tumor patients using a highly sensitive antibody immunohistochemistry assay |
title_sort | detection of braf mutation in chinese tumor patients using a highly sensitive antibody immunohistochemistry assay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363828/ https://www.ncbi.nlm.nih.gov/pubmed/25784606 http://dx.doi.org/10.1038/srep09211 |
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