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Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro
Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taisha...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363904/ https://www.ncbi.nlm.nih.gov/pubmed/25782009 http://dx.doi.org/10.1371/journal.pone.0115638 |
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author | Yang, Shifa Wei, Kai Jia, Fengjuan Zhao, Xue Cui, Guolin Guo, Fanxia Zhu, Ruiliang |
author_facet | Yang, Shifa Wei, Kai Jia, Fengjuan Zhao, Xue Cui, Guolin Guo, Fanxia Zhu, Ruiliang |
author_sort | Yang, Shifa |
collection | PubMed |
description | Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation. Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography. The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively. Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2. TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion. By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity. TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro. Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3. Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator. |
format | Online Article Text |
id | pubmed-4363904 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43639042015-03-23 Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro Yang, Shifa Wei, Kai Jia, Fengjuan Zhao, Xue Cui, Guolin Guo, Fanxia Zhu, Ruiliang PLoS One Research Article Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation. Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography. The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively. Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2. TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion. By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity. TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro. Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3. Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator. Public Library of Science 2015-03-17 /pmc/articles/PMC4363904/ /pubmed/25782009 http://dx.doi.org/10.1371/journal.pone.0115638 Text en © 2015 Yang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yang, Shifa Wei, Kai Jia, Fengjuan Zhao, Xue Cui, Guolin Guo, Fanxia Zhu, Ruiliang Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro |
title | Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro
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title_full | Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro
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title_fullStr | Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro
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title_full_unstemmed | Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro
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title_short | Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro
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title_sort | characterization and biological activity of taishan pinus massoniana pollen polysaccharide in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363904/ https://www.ncbi.nlm.nih.gov/pubmed/25782009 http://dx.doi.org/10.1371/journal.pone.0115638 |
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