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Serum microRNA-205 as a novel biomarker for cervical cancer patients

OBJECTIVE: Serum microRNAs (miRNAs) are a novel class of diagnostic and prognostic biomarkers for numerous cancers. However, the level and clinical relevance of circulating miR-205 transcripts in human serum of cervical cancer patients are unclear. The purpose of this study was to determine serum mi...

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Autores principales: Ma, Quanhui, Wan, Guiping, Wang, Shuxia, Yang, Wanwei, Zhang, Jiaming, Yao, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364049/
https://www.ncbi.nlm.nih.gov/pubmed/25788864
http://dx.doi.org/10.1186/s12935-014-0081-0
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author Ma, Quanhui
Wan, Guiping
Wang, Shuxia
Yang, Wanwei
Zhang, Jiaming
Yao, Xiaoming
author_facet Ma, Quanhui
Wan, Guiping
Wang, Shuxia
Yang, Wanwei
Zhang, Jiaming
Yao, Xiaoming
author_sort Ma, Quanhui
collection PubMed
description OBJECTIVE: Serum microRNAs (miRNAs) are a novel class of diagnostic and prognostic biomarkers for numerous cancers. However, the level and clinical relevance of circulating miR-205 transcripts in human serum of cervical cancer patients are unclear. The purpose of this study was to determine serum miR-205 levels in cervical cancer patients and explore its association with clinicopathological factors and prognosis. METHODS: Serum miR-205 expression was investigated in 60 cervical cancer patients and 60 healthy normal controls by using real-time PCR. Correlations between miR-205 expression and the clinicopathological features and prognosis of cervical cancer patients were then evaluated. Receiver operating characteristic curves were used to evaluate the sensitivity and specificity of serum miR-205. RESULTS: Serum miR-205 was significantly upregulated in cervical cancer patients compared with healthy donors (p < 0.01), and a high level of miR-205 expression was correlated with poor tumor differentiation (p = 0.009), lymph node metastasis (p = 0.015) and increased tumor stage (p = 0.001). The serum miR-205 level was capable of separating advanced stage from early stage metastatic cervical cancer from non-metastatic samples and poorly differentiated tumors from differentiated tumors with an area under the curve values of 0.74, 0.694 and 0.717, respectively. The expression of miR-205 was also higher in the cervical cancer tissues compared with the para-carcinoma tissues. In addition, Kaplan-Meier survival analysis showed that cervical cancer patients with high miR-205 expression tended to have shorter overall survival. In multivariate Cox regression analysis, miR-205 was identified as an independent prognostic marker. CONCLUSIONS: Serum miR-205, which is upregulated in cervical cancer, represents a predictive biomarker for the prognosis of cervical cancer patients.
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spelling pubmed-43640492015-03-19 Serum microRNA-205 as a novel biomarker for cervical cancer patients Ma, Quanhui Wan, Guiping Wang, Shuxia Yang, Wanwei Zhang, Jiaming Yao, Xiaoming Cancer Cell Int Primary Research OBJECTIVE: Serum microRNAs (miRNAs) are a novel class of diagnostic and prognostic biomarkers for numerous cancers. However, the level and clinical relevance of circulating miR-205 transcripts in human serum of cervical cancer patients are unclear. The purpose of this study was to determine serum miR-205 levels in cervical cancer patients and explore its association with clinicopathological factors and prognosis. METHODS: Serum miR-205 expression was investigated in 60 cervical cancer patients and 60 healthy normal controls by using real-time PCR. Correlations between miR-205 expression and the clinicopathological features and prognosis of cervical cancer patients were then evaluated. Receiver operating characteristic curves were used to evaluate the sensitivity and specificity of serum miR-205. RESULTS: Serum miR-205 was significantly upregulated in cervical cancer patients compared with healthy donors (p < 0.01), and a high level of miR-205 expression was correlated with poor tumor differentiation (p = 0.009), lymph node metastasis (p = 0.015) and increased tumor stage (p = 0.001). The serum miR-205 level was capable of separating advanced stage from early stage metastatic cervical cancer from non-metastatic samples and poorly differentiated tumors from differentiated tumors with an area under the curve values of 0.74, 0.694 and 0.717, respectively. The expression of miR-205 was also higher in the cervical cancer tissues compared with the para-carcinoma tissues. In addition, Kaplan-Meier survival analysis showed that cervical cancer patients with high miR-205 expression tended to have shorter overall survival. In multivariate Cox regression analysis, miR-205 was identified as an independent prognostic marker. CONCLUSIONS: Serum miR-205, which is upregulated in cervical cancer, represents a predictive biomarker for the prognosis of cervical cancer patients. BioMed Central 2014-08-22 /pmc/articles/PMC4364049/ /pubmed/25788864 http://dx.doi.org/10.1186/s12935-014-0081-0 Text en Copyright © 2014 Ma et al.; licensee Springer http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Primary Research
Ma, Quanhui
Wan, Guiping
Wang, Shuxia
Yang, Wanwei
Zhang, Jiaming
Yao, Xiaoming
Serum microRNA-205 as a novel biomarker for cervical cancer patients
title Serum microRNA-205 as a novel biomarker for cervical cancer patients
title_full Serum microRNA-205 as a novel biomarker for cervical cancer patients
title_fullStr Serum microRNA-205 as a novel biomarker for cervical cancer patients
title_full_unstemmed Serum microRNA-205 as a novel biomarker for cervical cancer patients
title_short Serum microRNA-205 as a novel biomarker for cervical cancer patients
title_sort serum microrna-205 as a novel biomarker for cervical cancer patients
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364049/
https://www.ncbi.nlm.nih.gov/pubmed/25788864
http://dx.doi.org/10.1186/s12935-014-0081-0
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