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Leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways

The aim of the present study was to evaluate the effect of leucine treatment (0.30 mM) on muscle weight and signaling of myoproteins related to synthesis and degradation pathways of soleus muscle following seven days of complete sciatic nerve lesion. Wistar rats (n = 24) of 3–4 months of age (192 ±...

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Autores principales: Ribeiro, Carolina B., Christofoletti, Daiane C., Pezolato, Vitor A., de Cássia Marqueti Durigan, Rita, Prestes, Jonato, Tibana, Ramires A., Pereira, Elaine C. L., de Sousa Neto, Ivo V., Durigan, João L. Q., da Silva, Carlos A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364154/
https://www.ncbi.nlm.nih.gov/pubmed/25852565
http://dx.doi.org/10.3389/fphys.2015.00073
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author Ribeiro, Carolina B.
Christofoletti, Daiane C.
Pezolato, Vitor A.
de Cássia Marqueti Durigan, Rita
Prestes, Jonato
Tibana, Ramires A.
Pereira, Elaine C. L.
de Sousa Neto, Ivo V.
Durigan, João L. Q.
da Silva, Carlos A.
author_facet Ribeiro, Carolina B.
Christofoletti, Daiane C.
Pezolato, Vitor A.
de Cássia Marqueti Durigan, Rita
Prestes, Jonato
Tibana, Ramires A.
Pereira, Elaine C. L.
de Sousa Neto, Ivo V.
Durigan, João L. Q.
da Silva, Carlos A.
author_sort Ribeiro, Carolina B.
collection PubMed
description The aim of the present study was to evaluate the effect of leucine treatment (0.30 mM) on muscle weight and signaling of myoproteins related to synthesis and degradation pathways of soleus muscle following seven days of complete sciatic nerve lesion. Wistar rats (n = 24) of 3–4 months of age (192 ± 23 g) were used. The animals were randomly distributed into four experimental groups (n = 6/group): control, treated with leucine (L), denervated (D) and denervated treated with leucine (DL). Dependent measures were proteins levels of AKT, AMPK, mTOR, and ACC performed by Western blot. Leucine induced a reduction in the phosphorylation of AMPK (p < 0.05) by 16% in the L and by 68% in the DL groups as compared with control group. Denervation increased AMPK by 24% in the D group as compared with the control group (p < 0.05). AKT was also modulated by denervation and leucine treatment, highlighted by the elevation of AKT phosphorylation in the D (65%), L (98%) and DL (146%) groups as compared with the control group (p < 0.05). AKT phosphorylation was 49% higher in the D group as compared with the DL group. Furthermore, denervation decreased mTOR phosphorylation by 29% in the D group as compared with the control group. However, leucine treatment induced an increase of 49% in the phosphorylation of mTOR in the L group as compared with the control group, and an increase of 154% in the DL as compared with the D group (p < 0.05). ACC phosphorylation was 20% greater in the D group than the control group. Furthermore, ACC in the soleus was 22% lower in the in the L group and 50% lower in the DL group than the respective control group (p < 0.05). In conclusion, leucine treatment minimized the deleterious effects of denervation on rat soleus muscle by increasing anabolic (AKT and mTOR) and decreasing catabolic (AMPK) pathways. These results may be interesting for muscle recovery following acute denervation, which may contribute to musculoskeletal rehabilitation after denervation.
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spelling pubmed-43641542015-04-07 Leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways Ribeiro, Carolina B. Christofoletti, Daiane C. Pezolato, Vitor A. de Cássia Marqueti Durigan, Rita Prestes, Jonato Tibana, Ramires A. Pereira, Elaine C. L. de Sousa Neto, Ivo V. Durigan, João L. Q. da Silva, Carlos A. Front Physiol Physiology The aim of the present study was to evaluate the effect of leucine treatment (0.30 mM) on muscle weight and signaling of myoproteins related to synthesis and degradation pathways of soleus muscle following seven days of complete sciatic nerve lesion. Wistar rats (n = 24) of 3–4 months of age (192 ± 23 g) were used. The animals were randomly distributed into four experimental groups (n = 6/group): control, treated with leucine (L), denervated (D) and denervated treated with leucine (DL). Dependent measures were proteins levels of AKT, AMPK, mTOR, and ACC performed by Western blot. Leucine induced a reduction in the phosphorylation of AMPK (p < 0.05) by 16% in the L and by 68% in the DL groups as compared with control group. Denervation increased AMPK by 24% in the D group as compared with the control group (p < 0.05). AKT was also modulated by denervation and leucine treatment, highlighted by the elevation of AKT phosphorylation in the D (65%), L (98%) and DL (146%) groups as compared with the control group (p < 0.05). AKT phosphorylation was 49% higher in the D group as compared with the DL group. Furthermore, denervation decreased mTOR phosphorylation by 29% in the D group as compared with the control group. However, leucine treatment induced an increase of 49% in the phosphorylation of mTOR in the L group as compared with the control group, and an increase of 154% in the DL as compared with the D group (p < 0.05). ACC phosphorylation was 20% greater in the D group than the control group. Furthermore, ACC in the soleus was 22% lower in the in the L group and 50% lower in the DL group than the respective control group (p < 0.05). In conclusion, leucine treatment minimized the deleterious effects of denervation on rat soleus muscle by increasing anabolic (AKT and mTOR) and decreasing catabolic (AMPK) pathways. These results may be interesting for muscle recovery following acute denervation, which may contribute to musculoskeletal rehabilitation after denervation. Frontiers Media S.A. 2015-03-18 /pmc/articles/PMC4364154/ /pubmed/25852565 http://dx.doi.org/10.3389/fphys.2015.00073 Text en Copyright © 2015 Ribeiro, Christofoletti, Pezolato, de Cássia Marqueti Durigan, Prestes, Tibana, Pereira, de Sousa Neto, Durigan and da Silva. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Ribeiro, Carolina B.
Christofoletti, Daiane C.
Pezolato, Vitor A.
de Cássia Marqueti Durigan, Rita
Prestes, Jonato
Tibana, Ramires A.
Pereira, Elaine C. L.
de Sousa Neto, Ivo V.
Durigan, João L. Q.
da Silva, Carlos A.
Leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways
title Leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways
title_full Leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways
title_fullStr Leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways
title_full_unstemmed Leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways
title_short Leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways
title_sort leucine minimizes denervation-induced skeletal muscle atrophy of rats through akt/mtor signaling pathways
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364154/
https://www.ncbi.nlm.nih.gov/pubmed/25852565
http://dx.doi.org/10.3389/fphys.2015.00073
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