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Differential temporal expression of S100β in developing rat brain

Radial glial cells (RGs) originally considered to provide scaffold to the radially migrating neurons constitute a heterogeneous population of the regionally variable precursor cells that generate both neurons as well as glia depending upon the location and the timing of development. Hence specific i...

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Autores principales: Patro, Nisha, Naik, Aijaz, Patro, Ishan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364248/
https://www.ncbi.nlm.nih.gov/pubmed/25852479
http://dx.doi.org/10.3389/fncel.2015.00087
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author Patro, Nisha
Naik, Aijaz
Patro, Ishan K.
author_facet Patro, Nisha
Naik, Aijaz
Patro, Ishan K.
author_sort Patro, Nisha
collection PubMed
description Radial glial cells (RGs) originally considered to provide scaffold to the radially migrating neurons constitute a heterogeneous population of the regionally variable precursor cells that generate both neurons as well as glia depending upon the location and the timing of development. Hence specific immunohistochemical markers are required to specify their spatiotemporal location and fate in the neurogenic and gliogenic zones. We hypothesize S100β as a potential and unified marker for both primary and secondary progenitors. To achieve this, cryocut sections from rat brains of varied embryonic and postnatal ages were immunolabeled with a combination of antibodies, i.e., S100β + Nestin, Nestin + GFAP and S100β + GFAP. A large population of the primary and secondary progenitors, lining the VZ and SVZ, simultaneously co-expressed S100β and nestin establishing their progenitor nature. A downregulation of both S100β and nestin noticed by the end of the 1st postnatal week marks their differentiation towards neuronal or glial lineage. In view of the absence of co-expression of GFAP (glial fibrillary acidic protein) either with S100β or nestin, the suitability of accepting GFAP as an early marker of RG’s was eliminated. Thus the dynamic expression of S100β in both the neural stem cells (NSCs) and RGs during embryonic and early neonatal life is associated with its proliferative potential and migration of undifferentiated neuroblasts and astrocytes. Once they lose their potential for proliferation, the S100β expression is repressed with its reemergence in mature astrocytes. This study provides the first clear evidence of S100β expression throughout the period of neurogenesis and early gliogenesis, suggesting its suitability as a radial progenitor cell marker.
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spelling pubmed-43642482015-04-07 Differential temporal expression of S100β in developing rat brain Patro, Nisha Naik, Aijaz Patro, Ishan K. Front Cell Neurosci Neuroscience Radial glial cells (RGs) originally considered to provide scaffold to the radially migrating neurons constitute a heterogeneous population of the regionally variable precursor cells that generate both neurons as well as glia depending upon the location and the timing of development. Hence specific immunohistochemical markers are required to specify their spatiotemporal location and fate in the neurogenic and gliogenic zones. We hypothesize S100β as a potential and unified marker for both primary and secondary progenitors. To achieve this, cryocut sections from rat brains of varied embryonic and postnatal ages were immunolabeled with a combination of antibodies, i.e., S100β + Nestin, Nestin + GFAP and S100β + GFAP. A large population of the primary and secondary progenitors, lining the VZ and SVZ, simultaneously co-expressed S100β and nestin establishing their progenitor nature. A downregulation of both S100β and nestin noticed by the end of the 1st postnatal week marks their differentiation towards neuronal or glial lineage. In view of the absence of co-expression of GFAP (glial fibrillary acidic protein) either with S100β or nestin, the suitability of accepting GFAP as an early marker of RG’s was eliminated. Thus the dynamic expression of S100β in both the neural stem cells (NSCs) and RGs during embryonic and early neonatal life is associated with its proliferative potential and migration of undifferentiated neuroblasts and astrocytes. Once they lose their potential for proliferation, the S100β expression is repressed with its reemergence in mature astrocytes. This study provides the first clear evidence of S100β expression throughout the period of neurogenesis and early gliogenesis, suggesting its suitability as a radial progenitor cell marker. Frontiers Media S.A. 2015-03-18 /pmc/articles/PMC4364248/ /pubmed/25852479 http://dx.doi.org/10.3389/fncel.2015.00087 Text en Copyright © 2015 Patro, Naik and Patro. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Patro, Nisha
Naik, Aijaz
Patro, Ishan K.
Differential temporal expression of S100β in developing rat brain
title Differential temporal expression of S100β in developing rat brain
title_full Differential temporal expression of S100β in developing rat brain
title_fullStr Differential temporal expression of S100β in developing rat brain
title_full_unstemmed Differential temporal expression of S100β in developing rat brain
title_short Differential temporal expression of S100β in developing rat brain
title_sort differential temporal expression of s100β in developing rat brain
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364248/
https://www.ncbi.nlm.nih.gov/pubmed/25852479
http://dx.doi.org/10.3389/fncel.2015.00087
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