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Microchamber Device for Detection of Transporter Activity of Adherent Cells

We present a method to detect the transporter activity of intact adherent cells using a microchamber device. When adherent cells are seeded onto the poly-di-methyl siloxane substrate having microchambers with openings smaller than the size of a cell, the cells form a confluent layer that covers the...

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Detalles Bibliográficos
Autores principales: Tsugane, Mamiko, Uejima, Etsuko, Suzuki, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364289/
https://www.ncbi.nlm.nih.gov/pubmed/25853126
http://dx.doi.org/10.3389/fbioe.2015.00032
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author Tsugane, Mamiko
Uejima, Etsuko
Suzuki, Hiroaki
author_facet Tsugane, Mamiko
Uejima, Etsuko
Suzuki, Hiroaki
author_sort Tsugane, Mamiko
collection PubMed
description We present a method to detect the transporter activity of intact adherent cells using a microchamber device. When adherent cells are seeded onto the poly-di-methyl siloxane substrate having microchambers with openings smaller than the size of a cell, the cells form a confluent layer that covers the microchambers, creating minute, confined spaces. As substances exported across the cell membrane accumulate, transporter activity can be detected by observing the fluorescence intensity increase in the microchamber. We tested the microchamber device with HeLa cells over-expressing MDR1, an ATP-binding cassette transporter, and succeeded in detecting the transport of fluorescence-conjugated paclitaxel, the anti-cancer drug, at the single-cell level.
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spelling pubmed-43642892015-04-07 Microchamber Device for Detection of Transporter Activity of Adherent Cells Tsugane, Mamiko Uejima, Etsuko Suzuki, Hiroaki Front Bioeng Biotechnol Bioengineering and Biotechnology We present a method to detect the transporter activity of intact adherent cells using a microchamber device. When adherent cells are seeded onto the poly-di-methyl siloxane substrate having microchambers with openings smaller than the size of a cell, the cells form a confluent layer that covers the microchambers, creating minute, confined spaces. As substances exported across the cell membrane accumulate, transporter activity can be detected by observing the fluorescence intensity increase in the microchamber. We tested the microchamber device with HeLa cells over-expressing MDR1, an ATP-binding cassette transporter, and succeeded in detecting the transport of fluorescence-conjugated paclitaxel, the anti-cancer drug, at the single-cell level. Frontiers Media S.A. 2015-03-18 /pmc/articles/PMC4364289/ /pubmed/25853126 http://dx.doi.org/10.3389/fbioe.2015.00032 Text en Copyright © 2015 Tsugane, Uejima and Suzuki. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Tsugane, Mamiko
Uejima, Etsuko
Suzuki, Hiroaki
Microchamber Device for Detection of Transporter Activity of Adherent Cells
title Microchamber Device for Detection of Transporter Activity of Adherent Cells
title_full Microchamber Device for Detection of Transporter Activity of Adherent Cells
title_fullStr Microchamber Device for Detection of Transporter Activity of Adherent Cells
title_full_unstemmed Microchamber Device for Detection of Transporter Activity of Adherent Cells
title_short Microchamber Device for Detection of Transporter Activity of Adherent Cells
title_sort microchamber device for detection of transporter activity of adherent cells
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364289/
https://www.ncbi.nlm.nih.gov/pubmed/25853126
http://dx.doi.org/10.3389/fbioe.2015.00032
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