Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats

Background. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents w...

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Autores principales: Kanamoto, Takashi, Souchelnytskyi, Nazariy, Kurimoto, Takuji, Ikeda, Yasuhiro, Sakaue, Hiroaki, Munemasa, Yasunari, Kiuchi, Yoshiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364380/
https://www.ncbi.nlm.nih.gov/pubmed/25821588
http://dx.doi.org/10.1155/2015/492050
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author Kanamoto, Takashi
Souchelnytskyi, Nazariy
Kurimoto, Takuji
Ikeda, Yasuhiro
Sakaue, Hiroaki
Munemasa, Yasunari
Kiuchi, Yoshiaki
author_facet Kanamoto, Takashi
Souchelnytskyi, Nazariy
Kurimoto, Takuji
Ikeda, Yasuhiro
Sakaue, Hiroaki
Munemasa, Yasunari
Kiuchi, Yoshiaki
author_sort Kanamoto, Takashi
collection PubMed
description Background. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents while the right eyes received no treatment and served as controls. After TES, the eye was enucleated and the retina was isolated. The retinas were analyzed by proteomics. Results. Proteomics showed that twenty-five proteins were upregulated by TES. The identified proteins included cellular signaling proteins, proteins associated with neuronal transmission, metabolic proteins, immunological factors, and structural proteins. Conclusions. TES induced changes in expression of various functional proteins in the retina.
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spelling pubmed-43643802015-03-29 Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats Kanamoto, Takashi Souchelnytskyi, Nazariy Kurimoto, Takuji Ikeda, Yasuhiro Sakaue, Hiroaki Munemasa, Yasunari Kiuchi, Yoshiaki J Ophthalmol Research Article Background. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents while the right eyes received no treatment and served as controls. After TES, the eye was enucleated and the retina was isolated. The retinas were analyzed by proteomics. Results. Proteomics showed that twenty-five proteins were upregulated by TES. The identified proteins included cellular signaling proteins, proteins associated with neuronal transmission, metabolic proteins, immunological factors, and structural proteins. Conclusions. TES induced changes in expression of various functional proteins in the retina. Hindawi Publishing Corporation 2015 2015-03-04 /pmc/articles/PMC4364380/ /pubmed/25821588 http://dx.doi.org/10.1155/2015/492050 Text en Copyright © 2015 Takashi Kanamoto et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kanamoto, Takashi
Souchelnytskyi, Nazariy
Kurimoto, Takuji
Ikeda, Yasuhiro
Sakaue, Hiroaki
Munemasa, Yasunari
Kiuchi, Yoshiaki
Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_full Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_fullStr Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_full_unstemmed Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_short Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats
title_sort proteomic study of retinal proteins associated with transcorneal electric stimulation in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364380/
https://www.ncbi.nlm.nih.gov/pubmed/25821588
http://dx.doi.org/10.1155/2015/492050
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