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A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human

Aging is defined as the accumulation of progressive organ dysfunction. Controlling the rate of aging by clarifying the complex pathways has a significant clinical importance. Nowadays, sirtuins have become famous molecules for slowing aging and decreasing age-related disorders. In the present study,...

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Autores principales: Kilic, Ulkan, Gok, Ozlem, Erenberk, Ufuk, Dundaroz, Mehmet Rusen, Torun, Emel, Kucukardali, Yasar, Elibol-Can, Birsen, Uysal, Omer, Dundar, Tolga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365019/
https://www.ncbi.nlm.nih.gov/pubmed/25785999
http://dx.doi.org/10.1371/journal.pone.0117954
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author Kilic, Ulkan
Gok, Ozlem
Erenberk, Ufuk
Dundaroz, Mehmet Rusen
Torun, Emel
Kucukardali, Yasar
Elibol-Can, Birsen
Uysal, Omer
Dundar, Tolga
author_facet Kilic, Ulkan
Gok, Ozlem
Erenberk, Ufuk
Dundaroz, Mehmet Rusen
Torun, Emel
Kucukardali, Yasar
Elibol-Can, Birsen
Uysal, Omer
Dundar, Tolga
author_sort Kilic, Ulkan
collection PubMed
description Aging is defined as the accumulation of progressive organ dysfunction. Controlling the rate of aging by clarifying the complex pathways has a significant clinical importance. Nowadays, sirtuins have become famous molecules for slowing aging and decreasing age-related disorders. In the present study, we analyzed the SIRT1 gene polymorphisms (rs7895833 A>G, rs7069102 C>G and rs2273773 C>T) and its relation with levels of SIRT1, eNOS, PON-1, cholesterol, TAS, TOS, and OSI to demonstrate the association between genetic variation in SIRT1 and phenotype at different ages in humans. We observed a significant increase in the SIRT1 level in older people and found a significant positive correlation between SIRT1 level and age in the overall studied population. The oldest people carrying AG genotypes for rs7895833 have the highest SIRT1 level suggesting an association between rs7895833 SNP and lifespan longevity. Older people have lower PON-1 levels than those of adults and children which may explain the high levels of SIRT1 protein as a compensatory mechanism for oxidative stress in the elderly. The eNOS protein level was significantly decreased in older people as compared to adults. There was no significant difference in the eNOS level between older people and children. The current study is the first to demonstrate age-related changes in SIRT1 levels in humans and it is important for a much better molecular understanding of the role of the longevity gene SIRT1 and its protein product in aging. It is also the first study presenting the association between SIRT1 expression in older people and rs7895833 in SIRT1 gene.
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spelling pubmed-43650192015-03-23 A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human Kilic, Ulkan Gok, Ozlem Erenberk, Ufuk Dundaroz, Mehmet Rusen Torun, Emel Kucukardali, Yasar Elibol-Can, Birsen Uysal, Omer Dundar, Tolga PLoS One Research Article Aging is defined as the accumulation of progressive organ dysfunction. Controlling the rate of aging by clarifying the complex pathways has a significant clinical importance. Nowadays, sirtuins have become famous molecules for slowing aging and decreasing age-related disorders. In the present study, we analyzed the SIRT1 gene polymorphisms (rs7895833 A>G, rs7069102 C>G and rs2273773 C>T) and its relation with levels of SIRT1, eNOS, PON-1, cholesterol, TAS, TOS, and OSI to demonstrate the association between genetic variation in SIRT1 and phenotype at different ages in humans. We observed a significant increase in the SIRT1 level in older people and found a significant positive correlation between SIRT1 level and age in the overall studied population. The oldest people carrying AG genotypes for rs7895833 have the highest SIRT1 level suggesting an association between rs7895833 SNP and lifespan longevity. Older people have lower PON-1 levels than those of adults and children which may explain the high levels of SIRT1 protein as a compensatory mechanism for oxidative stress in the elderly. The eNOS protein level was significantly decreased in older people as compared to adults. There was no significant difference in the eNOS level between older people and children. The current study is the first to demonstrate age-related changes in SIRT1 levels in humans and it is important for a much better molecular understanding of the role of the longevity gene SIRT1 and its protein product in aging. It is also the first study presenting the association between SIRT1 expression in older people and rs7895833 in SIRT1 gene. Public Library of Science 2015-03-18 /pmc/articles/PMC4365019/ /pubmed/25785999 http://dx.doi.org/10.1371/journal.pone.0117954 Text en © 2015 Kilic et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kilic, Ulkan
Gok, Ozlem
Erenberk, Ufuk
Dundaroz, Mehmet Rusen
Torun, Emel
Kucukardali, Yasar
Elibol-Can, Birsen
Uysal, Omer
Dundar, Tolga
A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human
title A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human
title_full A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human
title_fullStr A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human
title_full_unstemmed A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human
title_short A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: SIRT1 Gene Variants and Longevity in Human
title_sort remarkable age-related increase in sirt1 protein expression against oxidative stress in elderly: sirt1 gene variants and longevity in human
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365019/
https://www.ncbi.nlm.nih.gov/pubmed/25785999
http://dx.doi.org/10.1371/journal.pone.0117954
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