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Mycobacterial DNA Extraction for Whole-Genome Sequencing from Early Positive Liquid (MGIT) Cultures
We developed a low-cost and reliable method of DNA extraction from as little as 1 ml of early positive mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-genome sequencing to identify mycobacterial species and predict antibiotic resistance in clinical samples. The DNA ext...
Autores principales: | Votintseva, Antonina A., Pankhurst, Louise J., Anson, Luke W., Morgan, Marcus R., Gascoyne-Binzi, Deborah, Walker, Timothy M., Quan, T. Phuong, Wyllie, David H., Del Ojo Elias, Carlos, Wilcox, Mark, Walker, A. Sarah, Peto, Tim E. A., Crook, Derrick W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365189/ https://www.ncbi.nlm.nih.gov/pubmed/25631807 http://dx.doi.org/10.1128/JCM.03073-14 |
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