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Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health

There is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests for early-stage rheumatoid arthritis (eRA) such as rheumatoid factor (RF) and anti–cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass sp...

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Autores principales: Ahmed, Usman, Anwar, Attia, Savage, Richard S., Costa, Matthew L., Mackay, Nicola, Filer, Andrew, Raza, Karim, Watts, Richard A., Winyard, Paul G., Tarr, Joanna, Haigh, Richard C., Thornalley, Paul J., Rabbani, Naila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365413/
https://www.ncbi.nlm.nih.gov/pubmed/25788417
http://dx.doi.org/10.1038/srep09259
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author Ahmed, Usman
Anwar, Attia
Savage, Richard S.
Costa, Matthew L.
Mackay, Nicola
Filer, Andrew
Raza, Karim
Watts, Richard A.
Winyard, Paul G.
Tarr, Joanna
Haigh, Richard C.
Thornalley, Paul J.
Rabbani, Naila
author_facet Ahmed, Usman
Anwar, Attia
Savage, Richard S.
Costa, Matthew L.
Mackay, Nicola
Filer, Andrew
Raza, Karim
Watts, Richard A.
Winyard, Paul G.
Tarr, Joanna
Haigh, Richard C.
Thornalley, Paul J.
Rabbani, Naila
author_sort Ahmed, Usman
collection PubMed
description There is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests for early-stage rheumatoid arthritis (eRA) such as rheumatoid factor (RF) and anti–cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass spectrometric methods to quantify citrullinated protein (CP) and free hydroxyproline in body fluids. We detected CP in the plasma of healthy subjects and surprisingly found that CP was increased in both patients with eOA and eRA whereas anti–CCP antibodies were predominantly present in eRA. A 4-class diagnostic algorithm combining plasma/serum CP, anti-CCP antibody and hydroxyproline applied to a cohort gave specific and sensitive detection and discrimination of eOA, eRA, other non-RA inflammatory joint diseases and good skeletal health. This provides a first-in-class plasma/serum-based biochemical assay for diagnosis and type discrimination of early-stage arthritis to facilitate improved treatment and patient outcomes, exploiting citrullinated protein and related differential autoimmunity.
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spelling pubmed-43654132015-03-31 Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health Ahmed, Usman Anwar, Attia Savage, Richard S. Costa, Matthew L. Mackay, Nicola Filer, Andrew Raza, Karim Watts, Richard A. Winyard, Paul G. Tarr, Joanna Haigh, Richard C. Thornalley, Paul J. Rabbani, Naila Sci Rep Article There is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests for early-stage rheumatoid arthritis (eRA) such as rheumatoid factor (RF) and anti–cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass spectrometric methods to quantify citrullinated protein (CP) and free hydroxyproline in body fluids. We detected CP in the plasma of healthy subjects and surprisingly found that CP was increased in both patients with eOA and eRA whereas anti–CCP antibodies were predominantly present in eRA. A 4-class diagnostic algorithm combining plasma/serum CP, anti-CCP antibody and hydroxyproline applied to a cohort gave specific and sensitive detection and discrimination of eOA, eRA, other non-RA inflammatory joint diseases and good skeletal health. This provides a first-in-class plasma/serum-based biochemical assay for diagnosis and type discrimination of early-stage arthritis to facilitate improved treatment and patient outcomes, exploiting citrullinated protein and related differential autoimmunity. Nature Publishing Group 2015-03-19 /pmc/articles/PMC4365413/ /pubmed/25788417 http://dx.doi.org/10.1038/srep09259 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ahmed, Usman
Anwar, Attia
Savage, Richard S.
Costa, Matthew L.
Mackay, Nicola
Filer, Andrew
Raza, Karim
Watts, Richard A.
Winyard, Paul G.
Tarr, Joanna
Haigh, Richard C.
Thornalley, Paul J.
Rabbani, Naila
Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
title Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
title_full Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
title_fullStr Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
title_full_unstemmed Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
title_short Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
title_sort biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365413/
https://www.ncbi.nlm.nih.gov/pubmed/25788417
http://dx.doi.org/10.1038/srep09259
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