The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression

The G9a/GLP complex mediates mono- and dimethylation of Lys9 of histone H3 at specific gene loci, which is associated with transcriptional repression. However, the molecular mechanism by which the G9a/GLP complex is targeted to the specific gene loci for H3K9 methylation is unclear. In this study, w...

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Detalles Bibliográficos
Autores principales: Bian, Chunjing, Chen, Qiang, Yu, Xiaochun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365668/
https://www.ncbi.nlm.nih.gov/pubmed/25789554
http://dx.doi.org/10.7554/eLife.05606
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author Bian, Chunjing
Chen, Qiang
Yu, Xiaochun
author_facet Bian, Chunjing
Chen, Qiang
Yu, Xiaochun
author_sort Bian, Chunjing
collection PubMed
description The G9a/GLP complex mediates mono- and dimethylation of Lys9 of histone H3 at specific gene loci, which is associated with transcriptional repression. However, the molecular mechanism by which the G9a/GLP complex is targeted to the specific gene loci for H3K9 methylation is unclear. In this study, with unbiased protein affinity purification, we found ZNF644 and WIZ as two core subunits in the G9a/GLP complex. ZNF644 and WIZ interact with the transcription activation domain of G9a and GLP, respectively. Moreover, both ZNF644 and WIZ contain multiple zinc finger motifs that recognize consensus DNA sequences. ZNF644 and WIZ target G9a and GLP to the chromatin and mediate the G9a/GLP complex-dependent H3K9 methylation as well as gene repression. Thus, our studies reveal two key subunits in the G9a/GLP complex that regulate the function of this histone methyltransferase complex. DOI: http://dx.doi.org/10.7554/eLife.05606.001
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spelling pubmed-43656682015-03-24 The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression Bian, Chunjing Chen, Qiang Yu, Xiaochun eLife Biochemistry The G9a/GLP complex mediates mono- and dimethylation of Lys9 of histone H3 at specific gene loci, which is associated with transcriptional repression. However, the molecular mechanism by which the G9a/GLP complex is targeted to the specific gene loci for H3K9 methylation is unclear. In this study, with unbiased protein affinity purification, we found ZNF644 and WIZ as two core subunits in the G9a/GLP complex. ZNF644 and WIZ interact with the transcription activation domain of G9a and GLP, respectively. Moreover, both ZNF644 and WIZ contain multiple zinc finger motifs that recognize consensus DNA sequences. ZNF644 and WIZ target G9a and GLP to the chromatin and mediate the G9a/GLP complex-dependent H3K9 methylation as well as gene repression. Thus, our studies reveal two key subunits in the G9a/GLP complex that regulate the function of this histone methyltransferase complex. DOI: http://dx.doi.org/10.7554/eLife.05606.001 eLife Sciences Publications, Ltd 2015-03-19 /pmc/articles/PMC4365668/ /pubmed/25789554 http://dx.doi.org/10.7554/eLife.05606 Text en © 2015, Bian et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry
Bian, Chunjing
Chen, Qiang
Yu, Xiaochun
The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression
title The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression
title_full The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression
title_fullStr The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression
title_full_unstemmed The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression
title_short The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression
title_sort zinc finger proteins znf644 and wiz regulate the g9a/glp complex for gene repression
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365668/
https://www.ncbi.nlm.nih.gov/pubmed/25789554
http://dx.doi.org/10.7554/eLife.05606
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