A simple bioluminescent method for measuring d-amino acid oxidase activity

d-Amino acid oxidase (DAO) plays important roles in regulating d-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from d-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity bas...

Descripción completa

Detalles Bibliográficos
Autores principales: Bailey, T. Spencer, Donor, Micah T., Naughton, Sean P., Pluth, Michael D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365669/
https://www.ncbi.nlm.nih.gov/pubmed/25408176
http://dx.doi.org/10.1039/c4cc08145e
Descripción
Sumario:d-Amino acid oxidase (DAO) plays important roles in regulating d-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from d-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of d-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form d-luciferin.

Ejemplares similares