A simple bioluminescent method for measuring d-amino acid oxidase activity

d-Amino acid oxidase (DAO) plays important roles in regulating d-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from d-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity bas...

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Detalles Bibliográficos
Autores principales: Bailey, T. Spencer, Donor, Micah T., Naughton, Sean P., Pluth, Michael D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365669/
https://www.ncbi.nlm.nih.gov/pubmed/25408176
http://dx.doi.org/10.1039/c4cc08145e
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author Bailey, T. Spencer
Donor, Micah T.
Naughton, Sean P.
Pluth, Michael D.
author_facet Bailey, T. Spencer
Donor, Micah T.
Naughton, Sean P.
Pluth, Michael D.
author_sort Bailey, T. Spencer
collection PubMed
description d-Amino acid oxidase (DAO) plays important roles in regulating d-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from d-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of d-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form d-luciferin.
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spelling pubmed-43656692015-03-27 A simple bioluminescent method for measuring d-amino acid oxidase activity Bailey, T. Spencer Donor, Micah T. Naughton, Sean P. Pluth, Michael D. Chem Commun (Camb) Chemistry d-Amino acid oxidase (DAO) plays important roles in regulating d-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from d-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of d-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form d-luciferin. Royal Society of Chemistry 2015-03-28 2014-11-14 /pmc/articles/PMC4365669/ /pubmed/25408176 http://dx.doi.org/10.1039/c4cc08145e Text en This journal is © The Royal Society of Chemistry 2014 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Bailey, T. Spencer
Donor, Micah T.
Naughton, Sean P.
Pluth, Michael D.
A simple bioluminescent method for measuring d-amino acid oxidase activity
title A simple bioluminescent method for measuring d-amino acid oxidase activity
title_full A simple bioluminescent method for measuring d-amino acid oxidase activity
title_fullStr A simple bioluminescent method for measuring d-amino acid oxidase activity
title_full_unstemmed A simple bioluminescent method for measuring d-amino acid oxidase activity
title_short A simple bioluminescent method for measuring d-amino acid oxidase activity
title_sort simple bioluminescent method for measuring d-amino acid oxidase activity
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365669/
https://www.ncbi.nlm.nih.gov/pubmed/25408176
http://dx.doi.org/10.1039/c4cc08145e
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