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shRNA Off-Target Effects In Vivo: Impaired Endogenous siRNA Expression and Spermatogenic Defects

RNA interference (RNAi) is widely used to determine the function of genes. We chose this approach to assess the collective function of the highly related reproductive homeobox 3 (Rhox3) gene paralogs. Using a Rhox3 short hairpin (sh) RNA with 100% complementarity to all 8 Rhox3 paralogs, expressed f...

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Detalles Bibliográficos
Autores principales:	Song, Hye-Won, Bettegowda, Anilkumar, Oliver, Daniel, Yan, Wei, Phan, Mimi H., de Rooij, Dirk G., Corbett, Mark A., Wilkinson, Miles F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366048/ https://www.ncbi.nlm.nih.gov/pubmed/25790000 http://dx.doi.org/10.1371/journal.pone.0118549

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