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Rapid and Liquid-Based Selection of Genetic Switches Using Nucleoside Kinase Fused with Aminoglycoside Phosphotransferase

The evolutionary design of genetic switches and circuits requires iterative rounds of positive (ON-) and negative (OFF-) selection. We previously reported a rapid OFF selection system based on the kinase activity of herpes simplex virus thymidine kinase (hsvTK) on the artificial mutator nucleoside d...

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Detalles Bibliográficos
Autores principales:	Tominaga, Masahiro, Ike, Kohei, Kawai-Noma, Shigeko, Saito, Kyoichi, Umeno, Daisuke
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366196/ https://www.ncbi.nlm.nih.gov/pubmed/25790096 http://dx.doi.org/10.1371/journal.pone.0120243

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