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High-Resolution Sequence-Function Mapping of Full-Length Proteins

Comprehensive sequence-function mapping involves detailing the fitness contribution of every possible single mutation to a gene by comparing the abundance of each library variant before and after selection for the phenotype of interest. Deep sequencing of library DNA allows frequency reconstruction...

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Detalles Bibliográficos
Autores principales:	Kowalsky, Caitlin A., Klesmith, Justin R., Stapleton, James A., Kelly, Vince, Reichkitzer, Nolan, Whitehead, Timothy A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366243/ https://www.ncbi.nlm.nih.gov/pubmed/25790064 http://dx.doi.org/10.1371/journal.pone.0118193

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