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Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics

We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functional mature adipocytes from PIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our underst...

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Autores principales: Suzuki, Masahiko, Tada, Asuka, Kanmani, Paulraj, Watanabe, Hitoshi, Aso, Hisashi, Suda, Yoshihito, Nochi, Tomonori, Miyazawa, Kenji, Yoda, Kazutoyo, He, Fang, Hosoda, Masataka, Saito, Tadao, Villena, Julio, Kitazawa, Haruki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366390/
https://www.ncbi.nlm.nih.gov/pubmed/25789857
http://dx.doi.org/10.1371/journal.pone.0119644
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author Suzuki, Masahiko
Tada, Asuka
Kanmani, Paulraj
Watanabe, Hitoshi
Aso, Hisashi
Suda, Yoshihito
Nochi, Tomonori
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Hosoda, Masataka
Saito, Tadao
Villena, Julio
Kitazawa, Haruki
author_facet Suzuki, Masahiko
Tada, Asuka
Kanmani, Paulraj
Watanabe, Hitoshi
Aso, Hisashi
Suda, Yoshihito
Nochi, Tomonori
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Hosoda, Masataka
Saito, Tadao
Villena, Julio
Kitazawa, Haruki
author_sort Suzuki, Masahiko
collection PubMed
description We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functional mature adipocytes from PIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer’s patches (PPs) immune-competent cells. We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20, Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered.
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spelling pubmed-43663902015-03-23 Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics Suzuki, Masahiko Tada, Asuka Kanmani, Paulraj Watanabe, Hitoshi Aso, Hisashi Suda, Yoshihito Nochi, Tomonori Miyazawa, Kenji Yoda, Kazutoyo He, Fang Hosoda, Masataka Saito, Tadao Villena, Julio Kitazawa, Haruki PLoS One Research Article We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functional mature adipocytes from PIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer’s patches (PPs) immune-competent cells. We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20, Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered. Public Library of Science 2015-03-19 /pmc/articles/PMC4366390/ /pubmed/25789857 http://dx.doi.org/10.1371/journal.pone.0119644 Text en © 2015 Suzuki et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Suzuki, Masahiko
Tada, Asuka
Kanmani, Paulraj
Watanabe, Hitoshi
Aso, Hisashi
Suda, Yoshihito
Nochi, Tomonori
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Hosoda, Masataka
Saito, Tadao
Villena, Julio
Kitazawa, Haruki
Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics
title Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics
title_full Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics
title_fullStr Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics
title_full_unstemmed Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics
title_short Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics
title_sort advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366390/
https://www.ncbi.nlm.nih.gov/pubmed/25789857
http://dx.doi.org/10.1371/journal.pone.0119644
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