Cargando…
Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics
We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functional mature adipocytes from PIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our underst...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366390/ https://www.ncbi.nlm.nih.gov/pubmed/25789857 http://dx.doi.org/10.1371/journal.pone.0119644 |
_version_ | 1782362361050431488 |
---|---|
author | Suzuki, Masahiko Tada, Asuka Kanmani, Paulraj Watanabe, Hitoshi Aso, Hisashi Suda, Yoshihito Nochi, Tomonori Miyazawa, Kenji Yoda, Kazutoyo He, Fang Hosoda, Masataka Saito, Tadao Villena, Julio Kitazawa, Haruki |
author_facet | Suzuki, Masahiko Tada, Asuka Kanmani, Paulraj Watanabe, Hitoshi Aso, Hisashi Suda, Yoshihito Nochi, Tomonori Miyazawa, Kenji Yoda, Kazutoyo He, Fang Hosoda, Masataka Saito, Tadao Villena, Julio Kitazawa, Haruki |
author_sort | Suzuki, Masahiko |
collection | PubMed |
description | We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functional mature adipocytes from PIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer’s patches (PPs) immune-competent cells. We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20, Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered. |
format | Online Article Text |
id | pubmed-4366390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43663902015-03-23 Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics Suzuki, Masahiko Tada, Asuka Kanmani, Paulraj Watanabe, Hitoshi Aso, Hisashi Suda, Yoshihito Nochi, Tomonori Miyazawa, Kenji Yoda, Kazutoyo He, Fang Hosoda, Masataka Saito, Tadao Villena, Julio Kitazawa, Haruki PLoS One Research Article We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functional mature adipocytes from PIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer’s patches (PPs) immune-competent cells. We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20, Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered. Public Library of Science 2015-03-19 /pmc/articles/PMC4366390/ /pubmed/25789857 http://dx.doi.org/10.1371/journal.pone.0119644 Text en © 2015 Suzuki et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Suzuki, Masahiko Tada, Asuka Kanmani, Paulraj Watanabe, Hitoshi Aso, Hisashi Suda, Yoshihito Nochi, Tomonori Miyazawa, Kenji Yoda, Kazutoyo He, Fang Hosoda, Masataka Saito, Tadao Villena, Julio Kitazawa, Haruki Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics |
title | Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics |
title_full | Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics |
title_fullStr | Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics |
title_full_unstemmed | Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics |
title_short | Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics |
title_sort | advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366390/ https://www.ncbi.nlm.nih.gov/pubmed/25789857 http://dx.doi.org/10.1371/journal.pone.0119644 |
work_keys_str_mv | AT suzukimasahiko advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT tadaasuka advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT kanmanipaulraj advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT watanabehitoshi advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT asohisashi advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT sudayoshihito advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT nochitomonori advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT miyazawakenji advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT yodakazutoyo advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT hefang advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT hosodamasataka advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT saitotadao advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT villenajulio advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics AT kitazawaharuki advancedapplicationofporcineintramuscularadipocytesforevaluatingantiadipogenicandantiinflammatoryactivitiesofimmunobiotics |