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Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature

BACKGROUND: Adipose tissue damage of cryopreserved fat after autologous fat transfer is inevitable in several processes of re-transplantation. This study aims to compare and analyze the survivability of adipocytes after thawing fat cryopreserved at -20℃ by using thawing methods used in clinics. METH...

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Autores principales: Hwang, So-Min, Lee, Jong-Seo, Kim, Hyung-Do, Jung, Yong-Hui, Kim, Hong-Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Plastic and Reconstructive Surgeons 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366694/
https://www.ncbi.nlm.nih.gov/pubmed/25798384
http://dx.doi.org/10.5999/aps.2015.42.2.143
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author Hwang, So-Min
Lee, Jong-Seo
Kim, Hyung-Do
Jung, Yong-Hui
Kim, Hong-Il
author_facet Hwang, So-Min
Lee, Jong-Seo
Kim, Hyung-Do
Jung, Yong-Hui
Kim, Hong-Il
author_sort Hwang, So-Min
collection PubMed
description BACKGROUND: Adipose tissue damage of cryopreserved fat after autologous fat transfer is inevitable in several processes of re-transplantation. This study aims to compare and analyze the survivability of adipocytes after thawing fat cryopreserved at -20℃ by using thawing methods used in clinics. METHODS: The survival rates of adipocytes in the following thawing groups were measured: natural thawing at 25℃ for 15 minutes; natural thawing at 25℃ for 5 minutes, followed by rapid thawing at 37℃ in a water bath for 5 minutes; and rapid thawing at 37℃ for 10 minutes in a water bath. The survival rates of adipocytes were assessed by measuring the volume of the fat layer in the top layers separated after centrifugation, counting the number of live adipocytes after staining with trypan blue, and measuring the activity of mitochondria in the adipocytes. RESULTS: In the group with rapid thawing for 10 minutes in a water bath, it was observed that the cell count of live adipocytes and the activity of the adipocyte mitochondria were significantly higher than in the other two groups (P<0.05). The volume of the fat layer separated by centrifugation was also measured to be higher, which was, however, not statistically significant. CONCLUSIONS: It was shown that the survival rate of adipocytes was higher when the frozen fat tissue was thawed rapidly at 37℃. It can thus be concluded that if fats thawed with this method are re-transplanted, the survival rate of cryopreserved fats in transplantation will be improved, and thus, the effect of autologous fat transfer will increase.
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spelling pubmed-43666942015-03-20 Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature Hwang, So-Min Lee, Jong-Seo Kim, Hyung-Do Jung, Yong-Hui Kim, Hong-Il Arch Plast Surg Original Article BACKGROUND: Adipose tissue damage of cryopreserved fat after autologous fat transfer is inevitable in several processes of re-transplantation. This study aims to compare and analyze the survivability of adipocytes after thawing fat cryopreserved at -20℃ by using thawing methods used in clinics. METHODS: The survival rates of adipocytes in the following thawing groups were measured: natural thawing at 25℃ for 15 minutes; natural thawing at 25℃ for 5 minutes, followed by rapid thawing at 37℃ in a water bath for 5 minutes; and rapid thawing at 37℃ for 10 minutes in a water bath. The survival rates of adipocytes were assessed by measuring the volume of the fat layer in the top layers separated after centrifugation, counting the number of live adipocytes after staining with trypan blue, and measuring the activity of mitochondria in the adipocytes. RESULTS: In the group with rapid thawing for 10 minutes in a water bath, it was observed that the cell count of live adipocytes and the activity of the adipocyte mitochondria were significantly higher than in the other two groups (P<0.05). The volume of the fat layer separated by centrifugation was also measured to be higher, which was, however, not statistically significant. CONCLUSIONS: It was shown that the survival rate of adipocytes was higher when the frozen fat tissue was thawed rapidly at 37℃. It can thus be concluded that if fats thawed with this method are re-transplanted, the survival rate of cryopreserved fats in transplantation will be improved, and thus, the effect of autologous fat transfer will increase. The Korean Society of Plastic and Reconstructive Surgeons 2015-03 2015-03-16 /pmc/articles/PMC4366694/ /pubmed/25798384 http://dx.doi.org/10.5999/aps.2015.42.2.143 Text en Copyright © 2015 The Korean Society of Plastic and Reconstructive Surgeons http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hwang, So-Min
Lee, Jong-Seo
Kim, Hyung-Do
Jung, Yong-Hui
Kim, Hong-Il
Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature
title Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature
title_full Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature
title_fullStr Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature
title_full_unstemmed Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature
title_short Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature
title_sort comparison of the viability of cryopreserved fat tissue in accordance with the thawing temperature
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366694/
https://www.ncbi.nlm.nih.gov/pubmed/25798384
http://dx.doi.org/10.5999/aps.2015.42.2.143
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