Cargando…

Lathyrus sativus transcriptome resistance response to Ascochyta lathyri investigated by deepSuperSAGE analysis

Lathyrus sativus (grass pea) is a temperate grain legume crop with a great potential for expansion in dry areas or zones that are becoming more drought-prone. It is also recognized as a potential source of resistance to several important diseases in legumes, such as ascochyta blight. Nevertheless, t...

Descripción completa

Detalles Bibliográficos
Autores principales: Almeida, Nuno F., Krezdorn, Nicolas, Rotter, Björn, Winter, Peter, Rubiales, Diego, Vaz Patto, Maria C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4367168/
https://www.ncbi.nlm.nih.gov/pubmed/25852725
http://dx.doi.org/10.3389/fpls.2015.00178
_version_ 1782362497109458944
author Almeida, Nuno F.
Krezdorn, Nicolas
Rotter, Björn
Winter, Peter
Rubiales, Diego
Vaz Patto, Maria C.
author_facet Almeida, Nuno F.
Krezdorn, Nicolas
Rotter, Björn
Winter, Peter
Rubiales, Diego
Vaz Patto, Maria C.
author_sort Almeida, Nuno F.
collection PubMed
description Lathyrus sativus (grass pea) is a temperate grain legume crop with a great potential for expansion in dry areas or zones that are becoming more drought-prone. It is also recognized as a potential source of resistance to several important diseases in legumes, such as ascochyta blight. Nevertheless, the lack of detailed genomic and/or transcriptomic information hampers further exploitation of grass pea resistance-related genes in precision breeding. To elucidate the pathways differentially regulated during ascochyta-grass pea interaction and to identify resistance candidate genes, we compared the early response of the leaf gene expression profile of a resistant L. sativus genotype to Ascochyta lathyri infection with a non-inoculated control sample from the same genotype employing deepSuperSAGE. This analysis generated 14.387 UniTags of which 95.7% mapped to a reference grass pea/rust interaction transcriptome. From the total mapped UniTags, 738 were significantly differentially expressed between control and inoculated leaves. The results indicate that several gene classes acting in different phases of the plant/pathogen interaction are involved in the L. sativus response to A. lathyri infection. Most notably a clear up-regulation of defense-related genes involved in and/or regulated by the ethylene pathway was observed. There was also evidence of alterations in cell wall metabolism indicated by overexpression of cellulose synthase and lignin biosynthesis genes. This first genome-wide overview of the gene expression profile of the L. sativus response to ascochyta infection delivered a valuable set of candidate resistance genes for future use in precision breeding.
format Online
Article
Text
id pubmed-4367168
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-43671682015-04-07 Lathyrus sativus transcriptome resistance response to Ascochyta lathyri investigated by deepSuperSAGE analysis Almeida, Nuno F. Krezdorn, Nicolas Rotter, Björn Winter, Peter Rubiales, Diego Vaz Patto, Maria C. Front Plant Sci Plant Science Lathyrus sativus (grass pea) is a temperate grain legume crop with a great potential for expansion in dry areas or zones that are becoming more drought-prone. It is also recognized as a potential source of resistance to several important diseases in legumes, such as ascochyta blight. Nevertheless, the lack of detailed genomic and/or transcriptomic information hampers further exploitation of grass pea resistance-related genes in precision breeding. To elucidate the pathways differentially regulated during ascochyta-grass pea interaction and to identify resistance candidate genes, we compared the early response of the leaf gene expression profile of a resistant L. sativus genotype to Ascochyta lathyri infection with a non-inoculated control sample from the same genotype employing deepSuperSAGE. This analysis generated 14.387 UniTags of which 95.7% mapped to a reference grass pea/rust interaction transcriptome. From the total mapped UniTags, 738 were significantly differentially expressed between control and inoculated leaves. The results indicate that several gene classes acting in different phases of the plant/pathogen interaction are involved in the L. sativus response to A. lathyri infection. Most notably a clear up-regulation of defense-related genes involved in and/or regulated by the ethylene pathway was observed. There was also evidence of alterations in cell wall metabolism indicated by overexpression of cellulose synthase and lignin biosynthesis genes. This first genome-wide overview of the gene expression profile of the L. sativus response to ascochyta infection delivered a valuable set of candidate resistance genes for future use in precision breeding. Frontiers Media S.A. 2015-03-20 /pmc/articles/PMC4367168/ /pubmed/25852725 http://dx.doi.org/10.3389/fpls.2015.00178 Text en Copyright © 2015 Almeida, Krezdorn, Rotter, Winter, Rubiales and Vaz Patto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Almeida, Nuno F.
Krezdorn, Nicolas
Rotter, Björn
Winter, Peter
Rubiales, Diego
Vaz Patto, Maria C.
Lathyrus sativus transcriptome resistance response to Ascochyta lathyri investigated by deepSuperSAGE analysis
title Lathyrus sativus transcriptome resistance response to Ascochyta lathyri investigated by deepSuperSAGE analysis
title_full Lathyrus sativus transcriptome resistance response to Ascochyta lathyri investigated by deepSuperSAGE analysis
title_fullStr Lathyrus sativus transcriptome resistance response to Ascochyta lathyri investigated by deepSuperSAGE analysis
title_full_unstemmed Lathyrus sativus transcriptome resistance response to Ascochyta lathyri investigated by deepSuperSAGE analysis
title_short Lathyrus sativus transcriptome resistance response to Ascochyta lathyri investigated by deepSuperSAGE analysis
title_sort lathyrus sativus transcriptome resistance response to ascochyta lathyri investigated by deepsupersage analysis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4367168/
https://www.ncbi.nlm.nih.gov/pubmed/25852725
http://dx.doi.org/10.3389/fpls.2015.00178
work_keys_str_mv AT almeidanunof lathyrussativustranscriptomeresistanceresponsetoascochytalathyriinvestigatedbydeepsupersageanalysis
AT krezdornnicolas lathyrussativustranscriptomeresistanceresponsetoascochytalathyriinvestigatedbydeepsupersageanalysis
AT rotterbjorn lathyrussativustranscriptomeresistanceresponsetoascochytalathyriinvestigatedbydeepsupersageanalysis
AT winterpeter lathyrussativustranscriptomeresistanceresponsetoascochytalathyriinvestigatedbydeepsupersageanalysis
AT rubialesdiego lathyrussativustranscriptomeresistanceresponsetoascochytalathyriinvestigatedbydeepsupersageanalysis
AT vazpattomariac lathyrussativustranscriptomeresistanceresponsetoascochytalathyriinvestigatedbydeepsupersageanalysis