Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii

A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp. stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for th...

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Detalles Bibliográficos
Autores principales: Feng, Min, Kong, Dezhao, Wang, Wenbing, Liu, Liqiang, Song, Shanshan, Xu, Chuanlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4367412/
https://www.ncbi.nlm.nih.gov/pubmed/25686315
http://dx.doi.org/10.3390/s150204291
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author Feng, Min
Kong, Dezhao
Wang, Wenbing
Liu, Liqiang
Song, Shanshan
Xu, Chuanlai
author_facet Feng, Min
Kong, Dezhao
Wang, Wenbing
Liu, Liqiang
Song, Shanshan
Xu, Chuanlai
author_sort Feng, Min
collection PubMed
description A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp. stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 10(5) cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples.
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spelling pubmed-43674122015-04-30 Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii Feng, Min Kong, Dezhao Wang, Wenbing Liu, Liqiang Song, Shanshan Xu, Chuanlai Sensors (Basel) Article A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection of Pantoea stewartii subsp. stewartii (Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 10(5) cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples. MDPI 2015-02-12 /pmc/articles/PMC4367412/ /pubmed/25686315 http://dx.doi.org/10.3390/s150204291 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feng, Min
Kong, Dezhao
Wang, Wenbing
Liu, Liqiang
Song, Shanshan
Xu, Chuanlai
Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii
title Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii
title_full Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii
title_fullStr Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii
title_full_unstemmed Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii
title_short Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii
title_sort development of an immunochromatographic strip for rapid detection of pantoea stewartii subsp. stewartii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4367412/
https://www.ncbi.nlm.nih.gov/pubmed/25686315
http://dx.doi.org/10.3390/s150204291
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