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Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma

Human papillomavirus (HPV) infection is one of the most important etiologic causes of oropharyngeal head and neck squamous cell carcinoma (HNSCC). Patients with HPV-positive HNSCC were reported to have a better clinical outcome than patients with HPV-negative cancers. However, little is known about...

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Autores principales: Partlová, Simona, Bouček, Jan, Kloudová, Kamila, Lukešová, Eva, Zábrodský, Michal, Grega, Marek, Fučíková, Jitka, Truxová, Iva, Tachezy, Ruth, Špíšek, Radek, Fialová, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368144/
https://www.ncbi.nlm.nih.gov/pubmed/25949860
http://dx.doi.org/10.4161/21624011.2014.965570
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author Partlová, Simona
Bouček, Jan
Kloudová, Kamila
Lukešová, Eva
Zábrodský, Michal
Grega, Marek
Fučíková, Jitka
Truxová, Iva
Tachezy, Ruth
Špíšek, Radek
Fialová, Anna
author_facet Partlová, Simona
Bouček, Jan
Kloudová, Kamila
Lukešová, Eva
Zábrodský, Michal
Grega, Marek
Fučíková, Jitka
Truxová, Iva
Tachezy, Ruth
Špíšek, Radek
Fialová, Anna
author_sort Partlová, Simona
collection PubMed
description Human papillomavirus (HPV) infection is one of the most important etiologic causes of oropharyngeal head and neck squamous cell carcinoma (HNSCC). Patients with HPV-positive HNSCC were reported to have a better clinical outcome than patients with HPV-negative cancers. However, little is known about the possible causes of different clinical outcomes. In this study, we analyzed a detailed immune profile of tumor samples from HNSCC patients with respect to their HPV status. We analyzed the characteristics of immune cell infiltrates, including the frequency and distribution of antigen-presenting cells and naïve, regulatory and effector T cells and the cytokine and chemokine levels in tumor tissue. There was a profound difference in the extent and characteristics of intratumoral immune cell infiltrates in HNSCC patients based on their HPV status. In contrast to HPV-negative tumor tissues, HPV-positive tumor samples showed significantly higher numbers of infiltrating IFNγ(+) CD8(+) T lymphocytes, IL-17(+) CD8(+) T lymphocytes, myeloid dendritic cells and proinflammatory chemokines. Furthermore, HPV-positive tumors had significantly lower expression of Cox-2 mRNA and higher expression of PD1 mRNA compared to HPV-negative tumors. The presence of a high level of intratumoral immune cell infiltrates might play a crucial role in the significantly better response of HPV-positive patients to standard therapy and their favorable clinical outcome. Furthermore, characterization of the HNSCC immune profile might be a valuable prognostic tool in addition to HPV status and might help identify novel targets for therapeutic strategies, including cancer immunotherapy.
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spelling pubmed-43681442016-01-30 Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma Partlová, Simona Bouček, Jan Kloudová, Kamila Lukešová, Eva Zábrodský, Michal Grega, Marek Fučíková, Jitka Truxová, Iva Tachezy, Ruth Špíšek, Radek Fialová, Anna Oncoimmunology Original Research Human papillomavirus (HPV) infection is one of the most important etiologic causes of oropharyngeal head and neck squamous cell carcinoma (HNSCC). Patients with HPV-positive HNSCC were reported to have a better clinical outcome than patients with HPV-negative cancers. However, little is known about the possible causes of different clinical outcomes. In this study, we analyzed a detailed immune profile of tumor samples from HNSCC patients with respect to their HPV status. We analyzed the characteristics of immune cell infiltrates, including the frequency and distribution of antigen-presenting cells and naïve, regulatory and effector T cells and the cytokine and chemokine levels in tumor tissue. There was a profound difference in the extent and characteristics of intratumoral immune cell infiltrates in HNSCC patients based on their HPV status. In contrast to HPV-negative tumor tissues, HPV-positive tumor samples showed significantly higher numbers of infiltrating IFNγ(+) CD8(+) T lymphocytes, IL-17(+) CD8(+) T lymphocytes, myeloid dendritic cells and proinflammatory chemokines. Furthermore, HPV-positive tumors had significantly lower expression of Cox-2 mRNA and higher expression of PD1 mRNA compared to HPV-negative tumors. The presence of a high level of intratumoral immune cell infiltrates might play a crucial role in the significantly better response of HPV-positive patients to standard therapy and their favorable clinical outcome. Furthermore, characterization of the HNSCC immune profile might be a valuable prognostic tool in addition to HPV status and might help identify novel targets for therapeutic strategies, including cancer immunotherapy. Taylor & Francis 2015-01-30 /pmc/articles/PMC4368144/ /pubmed/25949860 http://dx.doi.org/10.4161/21624011.2014.965570 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Original Research
Partlová, Simona
Bouček, Jan
Kloudová, Kamila
Lukešová, Eva
Zábrodský, Michal
Grega, Marek
Fučíková, Jitka
Truxová, Iva
Tachezy, Ruth
Špíšek, Radek
Fialová, Anna
Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma
title Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma
title_full Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma
title_fullStr Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma
title_full_unstemmed Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma
title_short Distinct patterns of intratumoral immune cell infiltrates in patients with HPV-associated compared to non-virally induced head and neck squamous cell carcinoma
title_sort distinct patterns of intratumoral immune cell infiltrates in patients with hpv-associated compared to non-virally induced head and neck squamous cell carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368144/
https://www.ncbi.nlm.nih.gov/pubmed/25949860
http://dx.doi.org/10.4161/21624011.2014.965570
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