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Immunomodulatory Cross-Talk between Conjunctival Goblet Cells and Dendritic Cells
Goblet cells are secretory epithelial cells of mucosal tissues that confer protection from environmental agents or pathogens via expression and secretion of soluble mucins. Loss of these cells is associated with several chronic inflammatory disorders of the mucosa. Although demonstrated to transfer...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368435/ https://www.ncbi.nlm.nih.gov/pubmed/25793763 http://dx.doi.org/10.1371/journal.pone.0120284 |
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author | Contreras-Ruiz, Laura Masli, Sharmila |
author_facet | Contreras-Ruiz, Laura Masli, Sharmila |
author_sort | Contreras-Ruiz, Laura |
collection | PubMed |
description | Goblet cells are secretory epithelial cells of mucosal tissues that confer protection from environmental agents or pathogens via expression and secretion of soluble mucins. Loss of these cells is associated with several chronic inflammatory disorders of the mucosa. Although demonstrated to transfer antigens from the luminal surface to stromal cells in the intestinal mucosa, it is not known if goblet cells contribute to the regulation of an immune response. In this study we report that similar to intestinal and respiratory mucosal epithelia, mouse ocular surface epithelia predominantly express the TGF-ß2 isoform. Specifically, we demonstrate the ability of goblet cells to express TGF-ß2 and increase it in response to Toll-Like Receptor 4 mediated stimulus in cultures. Goblet cells not only express TGF-ß2, but are also able to activate it in a thrombospondin-1 (TSP-1) dependent manner via their cell surface receptor CD36. Furthermore, goblet cell derived soluble factors that possibly include TGF-ß2, alter dendritic cell (DC) phenotype to a tolerogenic type by downregulating DC expression of MHC class II and co-stimulatory molecules CD80, CD86 and CD40. Thus our study demonstrates goblet cells as a cellular source of active TGF-ß2 in ocular mucosa and implicates their immunomodulatory function in maintaining mucosal immune homeostasis. |
format | Online Article Text |
id | pubmed-4368435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43684352015-03-27 Immunomodulatory Cross-Talk between Conjunctival Goblet Cells and Dendritic Cells Contreras-Ruiz, Laura Masli, Sharmila PLoS One Research Article Goblet cells are secretory epithelial cells of mucosal tissues that confer protection from environmental agents or pathogens via expression and secretion of soluble mucins. Loss of these cells is associated with several chronic inflammatory disorders of the mucosa. Although demonstrated to transfer antigens from the luminal surface to stromal cells in the intestinal mucosa, it is not known if goblet cells contribute to the regulation of an immune response. In this study we report that similar to intestinal and respiratory mucosal epithelia, mouse ocular surface epithelia predominantly express the TGF-ß2 isoform. Specifically, we demonstrate the ability of goblet cells to express TGF-ß2 and increase it in response to Toll-Like Receptor 4 mediated stimulus in cultures. Goblet cells not only express TGF-ß2, but are also able to activate it in a thrombospondin-1 (TSP-1) dependent manner via their cell surface receptor CD36. Furthermore, goblet cell derived soluble factors that possibly include TGF-ß2, alter dendritic cell (DC) phenotype to a tolerogenic type by downregulating DC expression of MHC class II and co-stimulatory molecules CD80, CD86 and CD40. Thus our study demonstrates goblet cells as a cellular source of active TGF-ß2 in ocular mucosa and implicates their immunomodulatory function in maintaining mucosal immune homeostasis. Public Library of Science 2015-03-20 /pmc/articles/PMC4368435/ /pubmed/25793763 http://dx.doi.org/10.1371/journal.pone.0120284 Text en © 2015 Contreras-Ruiz, Masli http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Contreras-Ruiz, Laura Masli, Sharmila Immunomodulatory Cross-Talk between Conjunctival Goblet Cells and Dendritic Cells |
title | Immunomodulatory Cross-Talk between Conjunctival Goblet Cells and Dendritic Cells |
title_full | Immunomodulatory Cross-Talk between Conjunctival Goblet Cells and Dendritic Cells |
title_fullStr | Immunomodulatory Cross-Talk between Conjunctival Goblet Cells and Dendritic Cells |
title_full_unstemmed | Immunomodulatory Cross-Talk between Conjunctival Goblet Cells and Dendritic Cells |
title_short | Immunomodulatory Cross-Talk between Conjunctival Goblet Cells and Dendritic Cells |
title_sort | immunomodulatory cross-talk between conjunctival goblet cells and dendritic cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368435/ https://www.ncbi.nlm.nih.gov/pubmed/25793763 http://dx.doi.org/10.1371/journal.pone.0120284 |
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