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Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications

Infections are frequent and very undesired occurrences after orthopedic procedures; furthermore, the growing concern caused by the rise in antibiotic resistance is progressively dwindling the efficacy of such drugs. Artificial bone graft materials could solve some of the problems associated with the...

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Autores principales: González-Sánchez, M. Isabel, Perni, Stefano, Tommasi, Giacomo, Morris, Nathanael Glyn, Hawkins, Karl, López-Cabarcos, Enrique, Prokopovich, Polina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368440/
https://www.ncbi.nlm.nih.gov/pubmed/25746278
http://dx.doi.org/10.1016/j.msec.2015.02.002
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author González-Sánchez, M. Isabel
Perni, Stefano
Tommasi, Giacomo
Morris, Nathanael Glyn
Hawkins, Karl
López-Cabarcos, Enrique
Prokopovich, Polina
author_facet González-Sánchez, M. Isabel
Perni, Stefano
Tommasi, Giacomo
Morris, Nathanael Glyn
Hawkins, Karl
López-Cabarcos, Enrique
Prokopovich, Polina
author_sort González-Sánchez, M. Isabel
collection PubMed
description Infections are frequent and very undesired occurrences after orthopedic procedures; furthermore, the growing concern caused by the rise in antibiotic resistance is progressively dwindling the efficacy of such drugs. Artificial bone graft materials could solve some of the problems associated with the gold standard use of natural bone graft such as limited bone material, pain at the donor site and rejections if donor tissue is used. We have previously described new acrylate base nanocomposite hydrogels as bone graft materials. In the present paper, we describe the integration of silver nanoparticles in the polymeric mineralized biomaterial to provide non-antibiotic antibacterial activity against Staphylococcus epidermidis and Methicillin-resistant Staphylococcus aureus. Two different crosslinking degrees were tested and the silver nanoparticles were integrated into the composite matrix by means of three different methods: entrapment in the polymeric hydrogel before the mineralization; diffusion during the process of calcium phosphate crystallization and adsorption post-mineralization. The latter being generally the most effective method of encapsulation; however, the adsorption of silver nanoparticles inside the pores of the biomaterial led to a decreasing antibacterial activity for adsorption time longer than 2 days.
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spelling pubmed-43684402015-05-01 Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications González-Sánchez, M. Isabel Perni, Stefano Tommasi, Giacomo Morris, Nathanael Glyn Hawkins, Karl López-Cabarcos, Enrique Prokopovich, Polina Mater Sci Eng C Mater Biol Appl Article Infections are frequent and very undesired occurrences after orthopedic procedures; furthermore, the growing concern caused by the rise in antibiotic resistance is progressively dwindling the efficacy of such drugs. Artificial bone graft materials could solve some of the problems associated with the gold standard use of natural bone graft such as limited bone material, pain at the donor site and rejections if donor tissue is used. We have previously described new acrylate base nanocomposite hydrogels as bone graft materials. In the present paper, we describe the integration of silver nanoparticles in the polymeric mineralized biomaterial to provide non-antibiotic antibacterial activity against Staphylococcus epidermidis and Methicillin-resistant Staphylococcus aureus. Two different crosslinking degrees were tested and the silver nanoparticles were integrated into the composite matrix by means of three different methods: entrapment in the polymeric hydrogel before the mineralization; diffusion during the process of calcium phosphate crystallization and adsorption post-mineralization. The latter being generally the most effective method of encapsulation; however, the adsorption of silver nanoparticles inside the pores of the biomaterial led to a decreasing antibacterial activity for adsorption time longer than 2 days. Elsevier 2015-05-01 /pmc/articles/PMC4368440/ /pubmed/25746278 http://dx.doi.org/10.1016/j.msec.2015.02.002 Text en © 2015 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
González-Sánchez, M. Isabel
Perni, Stefano
Tommasi, Giacomo
Morris, Nathanael Glyn
Hawkins, Karl
López-Cabarcos, Enrique
Prokopovich, Polina
Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications
title Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications
title_full Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications
title_fullStr Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications
title_full_unstemmed Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications
title_short Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications
title_sort silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368440/
https://www.ncbi.nlm.nih.gov/pubmed/25746278
http://dx.doi.org/10.1016/j.msec.2015.02.002
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