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A Method for Combining RNAscope In Situ Hybridization with Immunohistochemistry in Thick Free-Floating Brain Sections and Primary Neuronal Cultures

In situ hybridization (ISH) is an extremely useful tool for localizing gene expression and changes in expression to specific cell populations in tissue samples across numerous research fields. Typically, a research group will put forth significant effort to design, generate, validate and then utiliz...

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Detalles Bibliográficos
Autores principales: Grabinski, Tessa M., Kneynsberg, Andrew, Manfredsson, Fredric P., Kanaan, Nicholas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368734/
https://www.ncbi.nlm.nih.gov/pubmed/25794171
http://dx.doi.org/10.1371/journal.pone.0120120