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A Method for Combining RNAscope In Situ Hybridization with Immunohistochemistry in Thick Free-Floating Brain Sections and Primary Neuronal Cultures
In situ hybridization (ISH) is an extremely useful tool for localizing gene expression and changes in expression to specific cell populations in tissue samples across numerous research fields. Typically, a research group will put forth significant effort to design, generate, validate and then utiliz...
Autores principales: | Grabinski, Tessa M., Kneynsberg, Andrew, Manfredsson, Fredric P., Kanaan, Nicholas M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368734/ https://www.ncbi.nlm.nih.gov/pubmed/25794171 http://dx.doi.org/10.1371/journal.pone.0120120 |
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