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Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response
Salt stress is one of most serious limiting factors for crop growth and production. An isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach was used to analyze proteomic changes in rice shoots under salt stress in this study. A total of 56 proteins were significantly altered and 16...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368772/ https://www.ncbi.nlm.nih.gov/pubmed/25793471 http://dx.doi.org/10.1371/journal.pone.0120978 |
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author | Xu, Jianwen Lan, Hongxia Fang, Huimin Huang, Xi Zhang, Hongsheng Huang, Ji |
author_facet | Xu, Jianwen Lan, Hongxia Fang, Huimin Huang, Xi Zhang, Hongsheng Huang, Ji |
author_sort | Xu, Jianwen |
collection | PubMed |
description | Salt stress is one of most serious limiting factors for crop growth and production. An isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach was used to analyze proteomic changes in rice shoots under salt stress in this study. A total of 56 proteins were significantly altered and 16 of them were enriched in the pathways of photosynthesis, antioxidant and oxidative phosphorylation. Among these 16 proteins, peroxiredoxin Q and photosystem I subunit D were up-regulated, while thioredoxin M-like, thioredoxin x, thioredoxin peroxidase, glutathione S-transferase F3, PSI subunit H, light-harvesting antenna complex I subunits, chloroplast chaperonin, vacuolar ATP synthase subunit H, and ATP synthase delta chain were down-regulated. Moreover, physiological data including total antioxidant capacity, peroxiredoxin activity, chlorophyll a/b content, glutathione S-transferase activity, reduced glutathione content and ATPase activity were consistent with changes in the levels of these proteins. The levels of the mRNAs encoding these proteins were also analyzed by real-time quantitative reverse transcription PCR, and approximately 86% of the results were consistent with the iTRAQ data. Importantly, our data suggest the important role of PSI in balancing energy supply and ROS generation under salt stress. This study provides information for an improved understanding of the function of photosynthesis and PSI in the salt-stress response of rice. |
format | Online Article Text |
id | pubmed-4368772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43687722015-03-27 Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response Xu, Jianwen Lan, Hongxia Fang, Huimin Huang, Xi Zhang, Hongsheng Huang, Ji PLoS One Research Article Salt stress is one of most serious limiting factors for crop growth and production. An isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach was used to analyze proteomic changes in rice shoots under salt stress in this study. A total of 56 proteins were significantly altered and 16 of them were enriched in the pathways of photosynthesis, antioxidant and oxidative phosphorylation. Among these 16 proteins, peroxiredoxin Q and photosystem I subunit D were up-regulated, while thioredoxin M-like, thioredoxin x, thioredoxin peroxidase, glutathione S-transferase F3, PSI subunit H, light-harvesting antenna complex I subunits, chloroplast chaperonin, vacuolar ATP synthase subunit H, and ATP synthase delta chain were down-regulated. Moreover, physiological data including total antioxidant capacity, peroxiredoxin activity, chlorophyll a/b content, glutathione S-transferase activity, reduced glutathione content and ATPase activity were consistent with changes in the levels of these proteins. The levels of the mRNAs encoding these proteins were also analyzed by real-time quantitative reverse transcription PCR, and approximately 86% of the results were consistent with the iTRAQ data. Importantly, our data suggest the important role of PSI in balancing energy supply and ROS generation under salt stress. This study provides information for an improved understanding of the function of photosynthesis and PSI in the salt-stress response of rice. Public Library of Science 2015-03-20 /pmc/articles/PMC4368772/ /pubmed/25793471 http://dx.doi.org/10.1371/journal.pone.0120978 Text en © 2015 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xu, Jianwen Lan, Hongxia Fang, Huimin Huang, Xi Zhang, Hongsheng Huang, Ji Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response |
title | Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response |
title_full | Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response |
title_fullStr | Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response |
title_full_unstemmed | Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response |
title_short | Quantitative Proteomic Analysis of the Rice (Oryza sativa L.) Salt Response |
title_sort | quantitative proteomic analysis of the rice (oryza sativa l.) salt response |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368772/ https://www.ncbi.nlm.nih.gov/pubmed/25793471 http://dx.doi.org/10.1371/journal.pone.0120978 |
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