Cargando…

Usefulness of real time PCR to quantify parasite load in serum samples from chronic Chagas disease patients

BACKGROUND: Inconclusive results of serological diagnosis in Chagas disease have an important impact on blood banks worldwide, reflecting in the high number of discarded bags or in an increased transmission by blood transfusion. Molecular techniques such as qPCR have been used for diagnosis and to m...

Descripción completa

Detalles Bibliográficos
Autores principales:	Melo, Myllena F, Moreira, Otacilio C, Tenório, Priscila, Lorena, Virginia, Lorena-Rezende, Izaura, Júnior, Wilson Oliveira, Gomes, Yara, Britto, Constança
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369093/ https://www.ncbi.nlm.nih.gov/pubmed/25890282 http://dx.doi.org/10.1186/s13071-015-0770-0

Ejemplares similares

Exploring the parasite load and molecular diversity of Trypanosoma cruzi in patients with chronic Chagas disease from different regions of Brazil
por: Rodrigues-dos-Santos, Ícaro, et al.
Publicado: (2018)

Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
por: D’Ávila, Daniella Alchaar, et al.
Publicado: (2018)

High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
por: Valença-Barbosa, Carolina, et al.
Publicado: (2022)

Memory impairment in chronic experimental Chagas disease: Benznidazole therapy reversed cognitive deficit in association with reduction of parasite load and oxidative stress in the nervous tissue
por: Vilar-Pereira, Glaucia, et al.
Publicado: (2021)

Tumor Necrosis Factor Is a Therapeutic Target for Immunological Unbalance and Cardiac Abnormalities in Chronic Experimental Chagas' Heart Disease
por: Pereira, Isabela Resende, et al.
Publicado: (2014)

Combination Chemotherapy with Suboptimal Doses of Benznidazole and Pentoxifylline Sustains Partial Reversion of Experimental Chagas' Heart Disease
por: Vilar-Pereira, Glaucia, et al.
Publicado: (2016)

Validation of the NAT Chagas IVD Kit for the Detection and Quantification of Trypanosoma cruzi in Blood Samples of Patients with Chagas Disease
por: Moreira, Otacilio C., et al.
Publicado: (2023)

Revisiting Chagas' disease diagnostic strategies in light of different scenarios of Trypanosoma cruzi infection
por: Britto, Constança Carvalho
Publicado: (2022)

Establishment of a murine model of congenital toxoplasmosis and validation of a qPCR assay to assess the parasite load in maternal and fetal tissues
por: Souza, Jéssica S., et al.
Publicado: (2023)

Accurate Real-Time PCR Strategy for Monitoring Bloodstream Parasitic Loads in Chagas Disease Patients
por: Duffy, Tomas, et al.
Publicado: (2009)

Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors
por: Moreira, Otacilio C., et al.
Publicado: (2017)

SYBR Green-based Real-Time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases
por: Pita-Pereira, Daniela, et al.
Publicado: (2012)

HRM Accuracy and Limitations as a Species Typing Tool for Leishmania Parasites
por: Filgueira, Camila Patricio Braga, et al.
Publicado: (2023)

Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
por: Ribeiro-Romão, Raquel Peralva, et al.
Publicado: (2016)

Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis
por: Filgueira, Camila Patricio Braga, et al.
Publicado: (2020)

Biomarkers and Echocardiographic Predictors of Cardiovascular Outcome in Patients With Chronic Chagas Disease
por: Mendes, Veronica G., et al.
Publicado: (2023)

Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
por: Silva-dos-Santos, Danielle, et al.
Publicado: (2017)

Parasite load evaluation by qPCR and blood culture in Chagas disease and HIV co-infected patients under antiretroviral therapy
por: Marcon, Gláucia Elisete Barbosa, et al.
Publicado: (2022)

Association between Trypanosoma cruzi DTU TcII and chronic Chagas disease clinical presentation and outcome in an urban cohort in Brazil
por: Nielebock, Marco Antonio Prates, et al.
Publicado: (2020)

New approaches for the standardization and validation of a real-time qPCR assay using TaqMan probes for quantification of yellow fever virus on clinical samples with high quality parameters
por: Fernandes-Monteiro, Alice G, et al.
Publicado: (2015)

Parasitic Load Correlates With Left Ventricular Dysfunction in Patients With Chronic Chagas Cardiomyopathy
por: de Oliveira, Maykon Tavares, et al.
Publicado: (2021)

Host-Parasite Interactions in Chagas Disease: Genetically Unidentical Isolates of a Single Trypanosoma cruzi Strain Identified In Vitro via LSSP-PCR
por: Nogueira-Paiva, Nívia Carolina, et al.
Publicado: (2015)

Modulation of miR-145-5p and miR-146b-5p levels is linked to reduced parasite load in H9C2 Trypanosoma cruzi infected cardiomyoblasts
por: Farani, Priscila Silva Grijó, et al.
Publicado: (2022)

Treatment With Suboptimal Dose of Benznidazole Mitigates Immune Response Molecular Pathways in Mice With Chronic Chagas Cardiomyopathy
por: Farani, Priscila Silva Grijó, et al.
Publicado: (2021)

First description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, Brazil
por: de Araujo-Pereira, Thais, et al.
Publicado: (2017)

Physical Exercise Promotes a Reduction in Cardiac Fibrosis in the Chronic Indeterminate Form of Experimental Chagas Disease
por: Pedra-Rezende, Yasmin, et al.
Publicado: (2021)

The Parasite Load of Trypanosoma cruzi Modulates Feeding and Defecation Patterns of the Chagas Disease Vector Triatoma infestans
por: Chacón, Francisco, et al.
Publicado: (2022)

Pentoxifylline Reverses Chronic Experimental Chagasic Cardiomyopathy in Association with Repositioning of Abnormal CD8(+) T-Cell Response
por: Pereira, Isabela Resende, et al.
Publicado: (2015)

Sexual transmission of American trypanosomiasis in humans: a new potential pandemic route for Chagas parasites
por: Araujo, Perla F, et al.
Publicado: (2017)

A new Trypanosoma cruzi genotyping method enables high resolution evolutionary analyses
por: Probst, Christian Macagnan, et al.
Publicado: (2021)

Neglected Parasitic Infections in the United States: Chagas Disease
por: Montgomery, Susan P., et al.
Publicado: (2014)

Meiotic sex in Chagas disease parasite Trypanosoma cruzi
por: Schwabl, Philipp, et al.
Publicado: (2019)

An Update on the Knowledge of Parasite–Vector Interactions of Chagas Disease
por: Schaub, Günter A
Publicado: (2021)

Venom alkaloids against Chagas disease parasite: search for effective therapies
por: Silva, Rafael C. M. Costa, et al.
Publicado: (2020)

Trypanosoma cruzi: Evaluation of PCR as a Laboratory Tool to Follow up the Evolution of Parasite Load
por: FERRAZ, Fabiana Nabarro, et al.
Publicado: (2016)

Treatment with benznidazole and pentoxifylline regulates microRNA transcriptomic profile in a murine model of Chagas chronic cardiomyopathy
por: Silva Grijó Farani, Priscila, et al.
Publicado: (2023)

Molecular Diagnosis of Chagas Disease in Colombia: Parasitic Loads and Discrete Typing Units in Patients from Acute and Chronic Phases
por: Hernández, Carolina, et al.
Publicado: (2016)

Role of FAK signaling in chagasic cardiac hypertrophy
por: Tucci, Amanda R., et al.
Publicado: (2020)

Trypanosoma cruzi in the Chicken Model: Chagas-Like Heart Disease in the Absence of Parasitism
por: Teixeira, Antonio R. L., et al.
Publicado: (2011)

Chagas Disease Infection Reactivation after Heart Transplant
por: Moreira, Maria da Consolação Vieira, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_3vvb30bsq8ekc4seknbgf7l2ta