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Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis
BACKGROUND: Quantitative real-time PCR is a valuable tool for evaluating bacterial gene expression. However, in order to make best use of this method, endogenous reference genes for expression data normalisation must first be identified by carefully validating the stability of expression under exper...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369107/ https://www.ncbi.nlm.nih.gov/pubmed/25884823 http://dx.doi.org/10.1186/s13104-015-1045-8 |