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Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis

BACKGROUND: Quantitative real-time PCR is a valuable tool for evaluating bacterial gene expression. However, in order to make best use of this method, endogenous reference genes for expression data normalisation must first be identified by carefully validating the stability of expression under exper...

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Detalles Bibliográficos
Autores principales: Bujold, Adina R, MacInnes, Janet I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369107/
https://www.ncbi.nlm.nih.gov/pubmed/25884823
http://dx.doi.org/10.1186/s13104-015-1045-8