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Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers

BACKGROUND: Chondrocytes are the main cellular component of articular cartilage. In healthy tissue, they are embedded in a strong but elastic extracelluar matrix providing resistance against mechanical forces and friction for the joints. Osteoarthritic cartilage, however, disrupted by heavy strain,...

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Autores principales: Wehland, Markus, Aleshcheva, Ganna, Schulz, Herbert, Saar, Katrin, Hübner, Norbert, Hemmersbach, Ruth, Braun, Markus, Ma, Xiao, Frett, Timo, Warnke, Elisabeth, Riwaldt, Stefan, Pietsch, Jessica, Corydon, Thomas Juhl, Infanger, Manfred, Grimm, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369370/
https://www.ncbi.nlm.nih.gov/pubmed/25889719
http://dx.doi.org/10.1186/s12964-015-0095-9
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author Wehland, Markus
Aleshcheva, Ganna
Schulz, Herbert
Saar, Katrin
Hübner, Norbert
Hemmersbach, Ruth
Braun, Markus
Ma, Xiao
Frett, Timo
Warnke, Elisabeth
Riwaldt, Stefan
Pietsch, Jessica
Corydon, Thomas Juhl
Infanger, Manfred
Grimm, Daniela
author_facet Wehland, Markus
Aleshcheva, Ganna
Schulz, Herbert
Saar, Katrin
Hübner, Norbert
Hemmersbach, Ruth
Braun, Markus
Ma, Xiao
Frett, Timo
Warnke, Elisabeth
Riwaldt, Stefan
Pietsch, Jessica
Corydon, Thomas Juhl
Infanger, Manfred
Grimm, Daniela
author_sort Wehland, Markus
collection PubMed
description BACKGROUND: Chondrocytes are the main cellular component of articular cartilage. In healthy tissue, they are embedded in a strong but elastic extracelluar matrix providing resistance against mechanical forces and friction for the joints. Osteoarthritic cartilage, however, disrupted by heavy strain, has only very limited potential to heal. One future possibility to replace damaged cartilage might be the scaffold-free growth of chondrocytes in microgravity to form 3D aggregates. RESULTS: To prepare for this, we have conducted experiments during the 20th DLR parabolic flight campaign, where we fixed the cells after the first (1P) and the 31st parabola (31P). Furthermore, we subjected chondrocytes to isolated vibration and hypergravity conditions. Microarray and quantitative real time PCR analyses revealed that hypergravity regulated genes connected to cartilage integrity (BMP4, MMP3, MMP10, EDN1, WNT5A, BIRC3). Vibration was clearly detrimental to cartilage (upregulated inflammatory IL6 and IL8, downregulated growth factors EGF, VEGF, FGF17). The viability of the cells was not affected by the parabolic flight, but showed a significantly increased expression of anti-apoptotic genes after 31 parabolas. The IL-6 release of chondrocytes cultured under conditions of vibration was not changed, but hypergravity (1.8 g) induced a clear elevation of IL-6 protein in the supernatant compared with corresponding control samples. CONCLUSION: Taken together, this study provided new insights into the growth behavior of chondrocytes under short-term microgravity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12964-015-0095-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-43693702015-03-23 Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers Wehland, Markus Aleshcheva, Ganna Schulz, Herbert Saar, Katrin Hübner, Norbert Hemmersbach, Ruth Braun, Markus Ma, Xiao Frett, Timo Warnke, Elisabeth Riwaldt, Stefan Pietsch, Jessica Corydon, Thomas Juhl Infanger, Manfred Grimm, Daniela Cell Commun Signal Research BACKGROUND: Chondrocytes are the main cellular component of articular cartilage. In healthy tissue, they are embedded in a strong but elastic extracelluar matrix providing resistance against mechanical forces and friction for the joints. Osteoarthritic cartilage, however, disrupted by heavy strain, has only very limited potential to heal. One future possibility to replace damaged cartilage might be the scaffold-free growth of chondrocytes in microgravity to form 3D aggregates. RESULTS: To prepare for this, we have conducted experiments during the 20th DLR parabolic flight campaign, where we fixed the cells after the first (1P) and the 31st parabola (31P). Furthermore, we subjected chondrocytes to isolated vibration and hypergravity conditions. Microarray and quantitative real time PCR analyses revealed that hypergravity regulated genes connected to cartilage integrity (BMP4, MMP3, MMP10, EDN1, WNT5A, BIRC3). Vibration was clearly detrimental to cartilage (upregulated inflammatory IL6 and IL8, downregulated growth factors EGF, VEGF, FGF17). The viability of the cells was not affected by the parabolic flight, but showed a significantly increased expression of anti-apoptotic genes after 31 parabolas. The IL-6 release of chondrocytes cultured under conditions of vibration was not changed, but hypergravity (1.8 g) induced a clear elevation of IL-6 protein in the supernatant compared with corresponding control samples. CONCLUSION: Taken together, this study provided new insights into the growth behavior of chondrocytes under short-term microgravity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12964-015-0095-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-20 /pmc/articles/PMC4369370/ /pubmed/25889719 http://dx.doi.org/10.1186/s12964-015-0095-9 Text en © Wehland et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wehland, Markus
Aleshcheva, Ganna
Schulz, Herbert
Saar, Katrin
Hübner, Norbert
Hemmersbach, Ruth
Braun, Markus
Ma, Xiao
Frett, Timo
Warnke, Elisabeth
Riwaldt, Stefan
Pietsch, Jessica
Corydon, Thomas Juhl
Infanger, Manfred
Grimm, Daniela
Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers
title Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers
title_full Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers
title_fullStr Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers
title_full_unstemmed Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers
title_short Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers
title_sort differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369370/
https://www.ncbi.nlm.nih.gov/pubmed/25889719
http://dx.doi.org/10.1186/s12964-015-0095-9
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