Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts

BACKGROUND: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. METHODS: Methanol extract was obta...

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Autores principales: Martinez-Elizalde, Karla Stephanie, Jimenez-Estrada, Manuel, Flores, Cesar Mateo, Hernandez, Luis Barbo, Rosas-Lopez, Rocio, Duran-Diaz, Angel, Nieto-Yañez, Oscar J, Barbosa, Elizabeth, Rodriguez-Monroy, Marco Aurelio, Canales-Martinez, Margarita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369878/
https://www.ncbi.nlm.nih.gov/pubmed/25887560
http://dx.doi.org/10.1186/s12906-015-0602-y
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author Martinez-Elizalde, Karla Stephanie
Jimenez-Estrada, Manuel
Flores, Cesar Mateo
Hernandez, Luis Barbo
Rosas-Lopez, Rocio
Duran-Diaz, Angel
Nieto-Yañez, Oscar J
Barbosa, Elizabeth
Rodriguez-Monroy, Marco Aurelio
Canales-Martinez, Margarita
author_facet Martinez-Elizalde, Karla Stephanie
Jimenez-Estrada, Manuel
Flores, Cesar Mateo
Hernandez, Luis Barbo
Rosas-Lopez, Rocio
Duran-Diaz, Angel
Nieto-Yañez, Oscar J
Barbosa, Elizabeth
Rodriguez-Monroy, Marco Aurelio
Canales-Martinez, Margarita
author_sort Martinez-Elizalde, Karla Stephanie
collection PubMed
description BACKGROUND: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. METHODS: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. RESULTS: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC = 4 mg/mL) and Vibrio cholera (MIC = 4 mg/mL). The MeOH2 inhibited the DPPH radical (SC(50) = 69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD(50) = 22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. CONCLUSIONS: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit.
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spelling pubmed-43698782015-03-24 Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts Martinez-Elizalde, Karla Stephanie Jimenez-Estrada, Manuel Flores, Cesar Mateo Hernandez, Luis Barbo Rosas-Lopez, Rocio Duran-Diaz, Angel Nieto-Yañez, Oscar J Barbosa, Elizabeth Rodriguez-Monroy, Marco Aurelio Canales-Martinez, Margarita BMC Complement Altern Med Research Article BACKGROUND: The fruit of Cyrtocarpa procera is used to treat stomach diseases by people living in San Rafael, Coxcatlan, Puebla. This work investigated the antibacterial, antioxidant, cytotoxic and anti-inflammatory activities of the fruit produced by this species. METHODS: Methanol extract was obtained by maceration. After obtaining the methanol extract (MeOH1), methanol subextract (MeOH2) and hexane (H) were obtained. The antibacterial activities of MeOH1, MeOH2 and H were evaluated through disc-diffusion. The quenching of free radicals was evaluated by decolorizing a methanolic DPPH solution. The cytotoxic activity of MeOH2 was evaluated by in vitro assay system of growth inhibition of human cervical carcinoma cell line (CasKi). The IL-1β and TNF-α were determined through ELISA in the supernatants of the macrophage cell line (RAW 264.7). The MeOH2 subextract was separated by column chromatography, seventy-three fractions were collected. RESULTS: The Gram-positive and -negative bacteria examined were sensitive to MeOH1 and MeOH2; the MeOH2 was bactericidal toward Staphyloccocus aureus (MIC = 4 mg/mL) and Vibrio cholera (MIC = 4 mg/mL). The MeOH2 inhibited the DPPH radical (SC(50) = 69.7 μg/mL), but a cytotoxicity assay revealed that the extract is not toxic according to the National Cancer Institute (LD(50) = 22.03 μg/mL). The production of proinflammatory cytokines (IL- 1β and TNF- α) by LPS- stimulated macrophages was reduced after the treatments. The methanol extract contained various organic acids, such as citric acid, palmitic acid and α- linoleic acid. CONCLUSIONS: The fruits of Cyrtocarpa procera are employed to treat ailments such as diarrhea, in this study were demonstrated some biological activities involved in a bacterial infection. This is the first research about of the medicinal properties of C. procera fruit. BioMed Central 2015-03-21 /pmc/articles/PMC4369878/ /pubmed/25887560 http://dx.doi.org/10.1186/s12906-015-0602-y Text en © Martinez-Elizalde et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Martinez-Elizalde, Karla Stephanie
Jimenez-Estrada, Manuel
Flores, Cesar Mateo
Hernandez, Luis Barbo
Rosas-Lopez, Rocio
Duran-Diaz, Angel
Nieto-Yañez, Oscar J
Barbosa, Elizabeth
Rodriguez-Monroy, Marco Aurelio
Canales-Martinez, Margarita
Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts
title Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts
title_full Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts
title_fullStr Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts
title_full_unstemmed Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts
title_short Evaluation of the medicinal properties of Cyrtocarpa procera Kunth fruit extracts
title_sort evaluation of the medicinal properties of cyrtocarpa procera kunth fruit extracts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369878/
https://www.ncbi.nlm.nih.gov/pubmed/25887560
http://dx.doi.org/10.1186/s12906-015-0602-y
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