Cargando…

Improving the Thermostability and Optimal Temperature of a Lipase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Covalent Immobilization

A recombinant thermostable lipase (Pf2001Δ60) from the hyperthermophilic Archaeon Pyrococcus furiosus (PFUL) was immobilized by hydrophobic interaction on octyl-agarose (octyl PFUL) and by covalent bond on aldehyde activated-agarose in the presence of DTT at pH = 7.0 (one-point covalent attachment)...

Descripción completa

Detalles Bibliográficos
Autores principales:	Branco, Roberta V., Gutarra, Melissa L. E., Guisan, Jose M., Freire, Denise M. G., Almeida, Rodrigo V., Palomo, Jose M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369884/ https://www.ncbi.nlm.nih.gov/pubmed/25839031 http://dx.doi.org/10.1155/2015/250532

Ejemplares similares

Immobilization and Characterization of a Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus on Supports with Different Degrees of Hydrophobicity
por: Branco, Roberta Vieira, et al.
Publicado: (2010)

Experimental and computational analysis of the secretome of the hyperthermophilic archaeon Pyrococcus furiosus
por: Schmid, G., et al.
Publicado: (2013)

Mutational Analyses of the Enzymes Involved in the Metabolism of Hydrogen by the Hyperthermophilic Archaeon Pyrococcus furiosus
por: Schut, Gerrit J., et al.
Publicado: (2012)

Ethanol production by the hyperthermophilic archaeon Pyrococcus furiosus by expression of bacterial bifunctional alcohol dehydrogenases
por: Keller, Matthew W., et al.
Publicado: (2017)

Identification of Membrane Proteins in the Hyperthermophilic Archaeon Pyrococcus Furiosus Using Proteomics and Prediction Programs
por: Holden, James F., et al.
Publicado: (2001)

Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus
por: Lobasso, Simona, et al.
Publicado: (2012)

Random mutagenesis of the hyperthermophilic archaeon Pyrococcus furiosus using in vitro mariner transposition and natural transformation
por: Guschinskaya, Natalia, et al.
Publicado: (2016)

A Novel Single-Strand Specific 3′–5′ Exonuclease Found in the Hyperthermophilic Archaeon, Pyrococcus furiosus
por: Tori, Kazuo, et al.
Publicado: (2013)

Characterization of Ten Heterotetrameric NDP-Dependent Acyl-CoA Synthetases of the Hyperthermophilic Archaeon Pyrococcus furiosus
por: Scott, Joseph W., et al.
Publicado: (2014)

Structure of hyperthermophilic β-glucosidase from Pyrococcus furiosus
por: Kado, Yuji, et al.
Publicado: (2011)

Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100
por: Alquéres, Sylvia Maria Campbell, et al.
Publicado: (2011)

Crystal structure of the ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus
por: Tatur, Jana, et al.
Publicado: (2007)

Proteome-Wide Prediction of Novel DNA/RNA-Binding Proteins Using Amino Acid Composition and Periodicity in the Hyperthermophilic Archaeon Pyrococcus furiosus
por: Fujishima, Kosuke, et al.
Publicado: (2007)

Intrinsic basis of thermostability of prolyl oligopeptidase from Pyrococcus furiosus
por: Banerjee, Sahini, et al.
Publicado: (2021)

Heterologous Production of an Energy-Conserving Carbon Monoxide Dehydrogenase Complex in the Hyperthermophile Pyrococcus furiosus
por: Schut, Gerrit J., et al.
Publicado: (2016)

Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
por: Swarts, Daan C., et al.
Publicado: (2015)

Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshii OT3
por: Usui, Kengo, et al.
Publicado: (2005)

Enhancing Heat Tolerance of the Little Dogwood Cornus canadensis L. f. with Introduction of a Superoxide Reductase Gene from the Hyperthermophilic Archaeon Pyrococcus furiosus
por: Geng, Xing-Min, et al.
Publicado: (2016)

Corrigendum: Enhancing Heat Tolerance of the Little Dogwood Cornus canadensis L. f. with Introduction of a Superoxide Reductase Gene from the Hyperthermophilic Archaeon Pyrococcus furiosus
por: Geng, Xing-Min, et al.
Publicado: (2016)

Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638
por: Denis, Antonia, et al.
Publicado: (2018)

Distant Non-Obvious Mutations Influence the Activity of a Hyperthermophilic Pyrococcus furiosus Phosphoglucose Isomerase
por: Subramanian, Kalyanasundaram, et al.
Publicado: (2019)

‘Pyrococcus furiosus, 30 years on’
por: Kengen, Servé W. M.
Publicado: (2017)

RNA at 92°C: The non-coding transcriptome of the hyperthermophilic archaeon Pyrococcus abyssi
por: Toffano-Nioche, Claire, et al.
Publicado: (2013)

Crystal structure of acylphosphatase from hyperthermophilic archaeon Pyrococcus horikoshii OT3
por: Miyazono, Ken-ichi, et al.
Publicado: (2004)

N-Linked Glycans Are Assembled on Highly Reduced Dolichol Phosphate Carriers in the Hyperthermophilic Archaea Pyrococcus furiosus
por: Chang, Michelle M., et al.
Publicado: (2015)

Structures of the Signal Recognition Particle Receptor from the Archaeon Pyrococcus furiosus: Implications for the Targeting Step at the Membrane
por: Egea, Pascal F., et al.
Publicado: (2008)

Operon prediction in Pyrococcus furiosus
por: Tran, Thao T., et al.
Publicado: (2007)

Preparation of lactose-free pasteurized milk with a recombinant thermostable β-glucosidase from Pyrococcus furiosus
por: Li, Bin, et al.
Publicado: (2013)

A hyper-thermostable α-amylase from Pyrococcus furiosus accumulates in Nicotiana tabacum as functional aggregates
por: Zhu, Hong, et al.
Publicado: (2017)

Primed CRISPR DNA uptake in Pyrococcus furiosus
por: Garrett, Sandra, et al.
Publicado: (2020)

Hyperthermophile Protein Behavior: Partially-Structured Conformations of Pyrococcus furiosus Rubredoxin Monomers Generated through Forced Cold-Denaturation and Refolding
por: Chandrayan, Sanjeev Kumar, et al.
Publicado: (2014)

Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus
por: Hogrel, Gaëlle, et al.
Publicado: (2018)

A Novel PLP-Dependent Alanine/Serine Racemase From the Hyperthermophilic Archaeon Pyrococcus horikoshii OT-3
por: Kawakami, Ryushi, et al.
Publicado: (2018)

Catalysis of iron core formation in Pyrococcus furiosus ferritin
por: Honarmand Ebrahimi, Kourosh, et al.
Publicado: (2009)

Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery
por: Daum, Bertram, et al.
Publicado: (2017)

A thermostable hybrid cluster protein from Pyrococcus furiosus: effects of the loss of a three helix bundle subdomain
por: Overeijnder, Marieke L., et al.
Publicado: (2009)

Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus
por: Wu, Chang-Hao, et al.
Publicado: (2015)

An Integrative Genomic Island Affects the Adaptations of the Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii to High Temperature and High Hydrostatic Pressure
por: Li, Zhen, et al.
Publicado: (2016)

Three CRISPR-Cas immune effector complexes coexist in Pyrococcus furiosus
por: Majumdar, Sonali, et al.
Publicado: (2015)

The CRISPR-associated Csx1 protein of Pyrococcus furiosus is an adenosine-specific endoribonuclease
por: Sheppard, Nolan F., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_rfd5e2d70odeumrhkp265v0sia