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Use of Droplet Digital PCR for Estimation of Fish Abundance and Biomass in Environmental DNA Surveys

An environmental DNA (eDNA) analysis method has been recently developed to estimate the distribution of aquatic animals by quantifying the number of target DNA copies with quantitative real-time PCR (qPCR). A new quantitative PCR technology, droplet digital PCR (ddPCR), partitions PCR reactions into...

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Detalles Bibliográficos
Autores principales:	Doi, Hideyuki, Uchii, Kimiko, Takahara, Teruhiko, Matsuhashi, Saeko, Yamanaka, Hiroki, Minamoto, Toshifumi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370432/ https://www.ncbi.nlm.nih.gov/pubmed/25799582 http://dx.doi.org/10.1371/journal.pone.0122763

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