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Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor

Though nanomaterials are considered as drug carriers or imaging reagents targeting the central nervous system their cytotoxicity effect on neuronal cells has not been well studied. In this study, we treated PC12 cells, a model neuronal cell line, with a nanomaterial that is widely accepted for medic...

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Detalles Bibliográficos
Autores principales: Sun, Bo, Liu, Rui, Ye, Nan, Xiao, Zhong-Dang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370573/
https://www.ncbi.nlm.nih.gov/pubmed/25798908
http://dx.doi.org/10.1371/journal.pone.0121671
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author Sun, Bo
Liu, Rui
Ye, Nan
Xiao, Zhong-Dang
author_facet Sun, Bo
Liu, Rui
Ye, Nan
Xiao, Zhong-Dang
author_sort Sun, Bo
collection PubMed
description Though nanomaterials are considered as drug carriers or imaging reagents targeting the central nervous system their cytotoxicity effect on neuronal cells has not been well studied. In this study, we treated PC12 cells, a model neuronal cell line, with a nanomaterial that is widely accepted for medical use, superparamagnetic iron oxide nanoparticles (SPIONs). Our results suggest that, after treated with SPIONs, the expression pattern of the cellular miRNAs changed widely in PC12 cells. As potential miRNA targets, NMDAR, one of the candidate mRNAs that were selected using GO and KEGG pathway enrichment, was significantly down regulated by SPIONs treatment. We further illustrated that SPIONs may induce cell death through NMDAR suppression. This study revealed a NMDAR neurotoxic effect of SPIONs and provides a reliable approach for assessing the neurocytotoxic effects of nanomaterials based on the comprehensive annotation of miRNA profiling.
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spelling pubmed-43705732015-04-04 Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor Sun, Bo Liu, Rui Ye, Nan Xiao, Zhong-Dang PLoS One Research Article Though nanomaterials are considered as drug carriers or imaging reagents targeting the central nervous system their cytotoxicity effect on neuronal cells has not been well studied. In this study, we treated PC12 cells, a model neuronal cell line, with a nanomaterial that is widely accepted for medical use, superparamagnetic iron oxide nanoparticles (SPIONs). Our results suggest that, after treated with SPIONs, the expression pattern of the cellular miRNAs changed widely in PC12 cells. As potential miRNA targets, NMDAR, one of the candidate mRNAs that were selected using GO and KEGG pathway enrichment, was significantly down regulated by SPIONs treatment. We further illustrated that SPIONs may induce cell death through NMDAR suppression. This study revealed a NMDAR neurotoxic effect of SPIONs and provides a reliable approach for assessing the neurocytotoxic effects of nanomaterials based on the comprehensive annotation of miRNA profiling. Public Library of Science 2015-03-23 /pmc/articles/PMC4370573/ /pubmed/25798908 http://dx.doi.org/10.1371/journal.pone.0121671 Text en © 2015 Sun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sun, Bo
Liu, Rui
Ye, Nan
Xiao, Zhong-Dang
Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor
title Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor
title_full Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor
title_fullStr Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor
title_full_unstemmed Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor
title_short Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor
title_sort comprehensive evaluation of microrna expression profiling reveals the neural signaling specific cytotoxicity of superparamagnetic iron oxide nanoparticles (spions) through n-methyl-d-aspartate receptor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370573/
https://www.ncbi.nlm.nih.gov/pubmed/25798908
http://dx.doi.org/10.1371/journal.pone.0121671
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