SNP Marker Discovery in Koala TLR Genes

Toll-like receptors (TLRs) play a crucial role in the early defence against invading pathogens, yet our understanding of TLRs in marsupial immunity is limited. Here, we describe the characterisation of nine TLRs from a koala immune tissue transcriptome and one TLR from a draft sequence of the koala...

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Autores principales: Cui, Jian, Frankham, Greta J., Johnson, Rebecca N., Polkinghorne, Adam, Timms, Peter, O’Meally, Denis, Cheng, Yuanyuan, Belov, Katherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370664/
https://www.ncbi.nlm.nih.gov/pubmed/25799012
http://dx.doi.org/10.1371/journal.pone.0121068
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author Cui, Jian
Frankham, Greta J.
Johnson, Rebecca N.
Polkinghorne, Adam
Timms, Peter
O’Meally, Denis
Cheng, Yuanyuan
Belov, Katherine
author_facet Cui, Jian
Frankham, Greta J.
Johnson, Rebecca N.
Polkinghorne, Adam
Timms, Peter
O’Meally, Denis
Cheng, Yuanyuan
Belov, Katherine
author_sort Cui, Jian
collection PubMed
description Toll-like receptors (TLRs) play a crucial role in the early defence against invading pathogens, yet our understanding of TLRs in marsupial immunity is limited. Here, we describe the characterisation of nine TLRs from a koala immune tissue transcriptome and one TLR from a draft sequence of the koala genome and the subsequent development of an assay to study genetic diversity in these genes. We surveyed genetic diversity in 20 koalas from New South Wales, Australia and showed that one gene, TLR10 is monomorphic, while the other nine TLR genes have between two and 12 alleles. 40 SNPs (16 non-synonymous) were identified across the ten TLR genes. These markers provide a springboard to future studies on innate immunity in the koala, a species under threat from two major infectious diseases.
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spelling pubmed-43706642015-04-04 SNP Marker Discovery in Koala TLR Genes Cui, Jian Frankham, Greta J. Johnson, Rebecca N. Polkinghorne, Adam Timms, Peter O’Meally, Denis Cheng, Yuanyuan Belov, Katherine PLoS One Research Article Toll-like receptors (TLRs) play a crucial role in the early defence against invading pathogens, yet our understanding of TLRs in marsupial immunity is limited. Here, we describe the characterisation of nine TLRs from a koala immune tissue transcriptome and one TLR from a draft sequence of the koala genome and the subsequent development of an assay to study genetic diversity in these genes. We surveyed genetic diversity in 20 koalas from New South Wales, Australia and showed that one gene, TLR10 is monomorphic, while the other nine TLR genes have between two and 12 alleles. 40 SNPs (16 non-synonymous) were identified across the ten TLR genes. These markers provide a springboard to future studies on innate immunity in the koala, a species under threat from two major infectious diseases. Public Library of Science 2015-03-23 /pmc/articles/PMC4370664/ /pubmed/25799012 http://dx.doi.org/10.1371/journal.pone.0121068 Text en © 2015 Cui et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cui, Jian
Frankham, Greta J.
Johnson, Rebecca N.
Polkinghorne, Adam
Timms, Peter
O’Meally, Denis
Cheng, Yuanyuan
Belov, Katherine
SNP Marker Discovery in Koala TLR Genes
title SNP Marker Discovery in Koala TLR Genes
title_full SNP Marker Discovery in Koala TLR Genes
title_fullStr SNP Marker Discovery in Koala TLR Genes
title_full_unstemmed SNP Marker Discovery in Koala TLR Genes
title_short SNP Marker Discovery in Koala TLR Genes
title_sort snp marker discovery in koala tlr genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370664/
https://www.ncbi.nlm.nih.gov/pubmed/25799012
http://dx.doi.org/10.1371/journal.pone.0121068
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