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Expression and purification of functional human glycogen synthase-1:glycogenin-1 complex in insect cells

We report the successful expression and purification of functional human muscle glycogen synthase (GYS1) in complex with human glycogenin-1 (GN1). Stoichiometric GYS1:GN1 complex was produced by co-expression of GYS1 and GN1 using a bicistronic pFastBac™-Dual expression vector, followed by affinity...

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Detalles Bibliográficos
Autores principales:	Hunter, Roger W., Zeqiraj, Elton, Morrice, Nicholas, Sicheri, Frank, Sakamoto, Kei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Academic Press 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370744/ https://www.ncbi.nlm.nih.gov/pubmed/25527037 http://dx.doi.org/10.1016/j.pep.2014.12.007

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