Cargando…
Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer
A surface plasmon resonance (SPR) immunoassay with an immobilization of self-assembled molecular identification membrane for the detection of residual Clenbuterol Hydrochloride (CLB) in pork liver was systematically investigated and experimentally validated for its high performance. SPR immunoassay...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370770/ https://www.ncbi.nlm.nih.gov/pubmed/25799327 http://dx.doi.org/10.1371/journal.pone.0122005 |
_version_ | 1782362936808833024 |
---|---|
author | Hu, Jiandong Chen, Ruipeng Wang, Shun Wang, Tingting Zhao, Yuanyuan Li, Jianwei Hu, Xinran Liang, Hao Zhu, Juanhua Sun, Xiaohui Ma, Liuzheng Jiang, Min |
author_facet | Hu, Jiandong Chen, Ruipeng Wang, Shun Wang, Tingting Zhao, Yuanyuan Li, Jianwei Hu, Xinran Liang, Hao Zhu, Juanhua Sun, Xiaohui Ma, Liuzheng Jiang, Min |
author_sort | Hu, Jiandong |
collection | PubMed |
description | A surface plasmon resonance (SPR) immunoassay with an immobilization of self-assembled molecular identification membrane for the detection of residual Clenbuterol Hydrochloride (CLB) in pork liver was systematically investigated and experimentally validated for its high performance. SPR immunoassay with a regular competitive inhibition assay cannot be directly verified to detect CLB residuals. In this study, the binding of Au film with mercaptopropionic acid was investigated using the known form of the strong S-Au covalent bonds formed by the chemical radical of the mercaptopropionic acid and the Au film. After that, the immunoglobulin IgG of swine (SwIgG-CLB) was bonded with the mercaptopropionic acid by covalent -CO-NH- amide bonding. The modified comprehensive analysis of how the membrane structure works was introduced together with the customized SPR bioanalyzer. In order to evaluate the performance of this biomembrane structure, the concentrations of CLB-contained solutions of 0 ng•mL(-1), 10 ng•mL(-1), 20 ng•mL(-1), 33.3 ng•mL(-1), and 40 ng•mL(-1) were prepared by adding CLB reagents into the solutions of CLB antibody (Clenbuterol Hydrochloride Antibody, CLB-Ab), successively and then the response unit (RU) was measured individually. Using the data collected from the linear CCD array, the fitting curve was established with the R-Square value of 0.9929. Correspondingly, the recovery rate ranged from 88.48% to 103.21% was experimented and the limit of detection of CLB in 1.26 ng•mL(-1) was obtained efficiently. It was concluded that the detection method associated with biomembrane properties is expected to contribute much to the determination of residual CLB in pork liver quantitatively by using the customized SPR bioanalyzer. |
format | Online Article Text |
id | pubmed-4370770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43707702015-04-04 Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer Hu, Jiandong Chen, Ruipeng Wang, Shun Wang, Tingting Zhao, Yuanyuan Li, Jianwei Hu, Xinran Liang, Hao Zhu, Juanhua Sun, Xiaohui Ma, Liuzheng Jiang, Min PLoS One Research Article A surface plasmon resonance (SPR) immunoassay with an immobilization of self-assembled molecular identification membrane for the detection of residual Clenbuterol Hydrochloride (CLB) in pork liver was systematically investigated and experimentally validated for its high performance. SPR immunoassay with a regular competitive inhibition assay cannot be directly verified to detect CLB residuals. In this study, the binding of Au film with mercaptopropionic acid was investigated using the known form of the strong S-Au covalent bonds formed by the chemical radical of the mercaptopropionic acid and the Au film. After that, the immunoglobulin IgG of swine (SwIgG-CLB) was bonded with the mercaptopropionic acid by covalent -CO-NH- amide bonding. The modified comprehensive analysis of how the membrane structure works was introduced together with the customized SPR bioanalyzer. In order to evaluate the performance of this biomembrane structure, the concentrations of CLB-contained solutions of 0 ng•mL(-1), 10 ng•mL(-1), 20 ng•mL(-1), 33.3 ng•mL(-1), and 40 ng•mL(-1) were prepared by adding CLB reagents into the solutions of CLB antibody (Clenbuterol Hydrochloride Antibody, CLB-Ab), successively and then the response unit (RU) was measured individually. Using the data collected from the linear CCD array, the fitting curve was established with the R-Square value of 0.9929. Correspondingly, the recovery rate ranged from 88.48% to 103.21% was experimented and the limit of detection of CLB in 1.26 ng•mL(-1) was obtained efficiently. It was concluded that the detection method associated with biomembrane properties is expected to contribute much to the determination of residual CLB in pork liver quantitatively by using the customized SPR bioanalyzer. Public Library of Science 2015-03-23 /pmc/articles/PMC4370770/ /pubmed/25799327 http://dx.doi.org/10.1371/journal.pone.0122005 Text en © 2015 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hu, Jiandong Chen, Ruipeng Wang, Shun Wang, Tingting Zhao, Yuanyuan Li, Jianwei Hu, Xinran Liang, Hao Zhu, Juanhua Sun, Xiaohui Ma, Liuzheng Jiang, Min Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer |
title | Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer |
title_full | Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer |
title_fullStr | Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer |
title_full_unstemmed | Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer |
title_short | Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer |
title_sort | detection of clenbuterol hydrochloride residuals in pork liver using a customized surface plasmon resonance bioanalyzer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370770/ https://www.ncbi.nlm.nih.gov/pubmed/25799327 http://dx.doi.org/10.1371/journal.pone.0122005 |
work_keys_str_mv | AT hujiandong detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT chenruipeng detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT wangshun detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT wangtingting detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT zhaoyuanyuan detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT lijianwei detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT huxinran detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT lianghao detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT zhujuanhua detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT sunxiaohui detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT maliuzheng detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer AT jiangmin detectionofclenbuterolhydrochlorideresidualsinporkliverusingacustomizedsurfaceplasmonresonancebioanalyzer |