The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods

A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR to detect genes coding for the carbapenemases KPC, NDM, OXA-48, VIM, IMP a...

Descripción completa

Detalles Bibliográficos
Autores principales: van der Zwaluw, Kim, de Haan, Angela, Pluister, Gerlinde N., Bootsma, Hester J., de Neeling, Albert J., Schouls, Leo M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370852/
https://www.ncbi.nlm.nih.gov/pubmed/25798828
http://dx.doi.org/10.1371/journal.pone.0123690
_version_ 1782362945465876480
author van der Zwaluw, Kim
de Haan, Angela
Pluister, Gerlinde N.
Bootsma, Hester J.
de Neeling, Albert J.
Schouls, Leo M.
author_facet van der Zwaluw, Kim
de Haan, Angela
Pluister, Gerlinde N.
Bootsma, Hester J.
de Neeling, Albert J.
Schouls, Leo M.
author_sort van der Zwaluw, Kim
collection PubMed
description A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR to detect genes coding for the carbapenemases KPC, NDM, OXA-48, VIM, IMP and OXA-23. It allows reliable detection of carbapenemase activity encoded by various genes in species of Enterobacteriaceae (e.g., Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae), but also in non-fermenters Pseudomonas aeruginosa and Acinetobacter baumannii. The CIM was shown to be a cost-effective and highly robust phenotypic screening method that can reliably detect carbapenemase activity.
format Online
Article
Text
id pubmed-4370852
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-43708522015-04-04 The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods van der Zwaluw, Kim de Haan, Angela Pluister, Gerlinde N. Bootsma, Hester J. de Neeling, Albert J. Schouls, Leo M. PLoS One Research Article A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR to detect genes coding for the carbapenemases KPC, NDM, OXA-48, VIM, IMP and OXA-23. It allows reliable detection of carbapenemase activity encoded by various genes in species of Enterobacteriaceae (e.g., Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae), but also in non-fermenters Pseudomonas aeruginosa and Acinetobacter baumannii. The CIM was shown to be a cost-effective and highly robust phenotypic screening method that can reliably detect carbapenemase activity. Public Library of Science 2015-03-23 /pmc/articles/PMC4370852/ /pubmed/25798828 http://dx.doi.org/10.1371/journal.pone.0123690 Text en © 2015 van der Zwaluw et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
van der Zwaluw, Kim
de Haan, Angela
Pluister, Gerlinde N.
Bootsma, Hester J.
de Neeling, Albert J.
Schouls, Leo M.
The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods
title The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods
title_full The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods
title_fullStr The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods
title_full_unstemmed The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods
title_short The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods
title_sort carbapenem inactivation method (cim), a simple and low-cost alternative for the carba np test to assess phenotypic carbapenemase activity in gram-negative rods
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370852/
https://www.ncbi.nlm.nih.gov/pubmed/25798828
http://dx.doi.org/10.1371/journal.pone.0123690
work_keys_str_mv AT vanderzwaluwkim thecarbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT dehaanangela thecarbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT pluistergerlinden thecarbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT bootsmahesterj thecarbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT deneelingalbertj thecarbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT schoulsleom thecarbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT vanderzwaluwkim carbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT dehaanangela carbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT pluistergerlinden carbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT bootsmahesterj carbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT deneelingalbertj carbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods
AT schoulsleom carbapeneminactivationmethodcimasimpleandlowcostalternativeforthecarbanptesttoassessphenotypiccarbapenemaseactivityingramnegativerods

Ejemplares similares