Cargando…
Entrainment of heterogeneous glycolytic oscillations in single cells
Cell signaling, gene expression, and metabolism are affected by cell-cell heterogeneity and random changes in the environment. The effects of such fluctuations on cell signaling and gene expression have recently been studied intensively using single-cell experiments. In metabolism heterogeneity may...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371117/ https://www.ncbi.nlm.nih.gov/pubmed/25802053 http://dx.doi.org/10.1038/srep09404 |
_version_ | 1782362987978293248 |
---|---|
author | Gustavsson, Anna-Karin Adiels, Caroline B. Mehlig, Bernhard Goksör, Mattias |
author_facet | Gustavsson, Anna-Karin Adiels, Caroline B. Mehlig, Bernhard Goksör, Mattias |
author_sort | Gustavsson, Anna-Karin |
collection | PubMed |
description | Cell signaling, gene expression, and metabolism are affected by cell-cell heterogeneity and random changes in the environment. The effects of such fluctuations on cell signaling and gene expression have recently been studied intensively using single-cell experiments. In metabolism heterogeneity may be particularly important because it may affect synchronisation of metabolic oscillations, an important example of cell-cell communication. This synchronisation is notoriously difficult to describe theoretically as the example of glycolytic oscillations shows: neither is the mechanism of glycolytic synchronisation understood nor the role of cell-cell heterogeneity. To pin down the mechanism and to assess its robustness and universality we have experimentally investigated the entrainment of glycolytic oscillations in individual yeast cells by periodic external perturbations. We find that oscillatory cells synchronise through phase shifts and that the mechanism is insensitive to cell heterogeneity (robustness) and similar for different types of external perturbations (universality). |
format | Online Article Text |
id | pubmed-4371117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43711172015-04-06 Entrainment of heterogeneous glycolytic oscillations in single cells Gustavsson, Anna-Karin Adiels, Caroline B. Mehlig, Bernhard Goksör, Mattias Sci Rep Article Cell signaling, gene expression, and metabolism are affected by cell-cell heterogeneity and random changes in the environment. The effects of such fluctuations on cell signaling and gene expression have recently been studied intensively using single-cell experiments. In metabolism heterogeneity may be particularly important because it may affect synchronisation of metabolic oscillations, an important example of cell-cell communication. This synchronisation is notoriously difficult to describe theoretically as the example of glycolytic oscillations shows: neither is the mechanism of glycolytic synchronisation understood nor the role of cell-cell heterogeneity. To pin down the mechanism and to assess its robustness and universality we have experimentally investigated the entrainment of glycolytic oscillations in individual yeast cells by periodic external perturbations. We find that oscillatory cells synchronise through phase shifts and that the mechanism is insensitive to cell heterogeneity (robustness) and similar for different types of external perturbations (universality). Nature Publishing Group 2015-03-24 /pmc/articles/PMC4371117/ /pubmed/25802053 http://dx.doi.org/10.1038/srep09404 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Gustavsson, Anna-Karin Adiels, Caroline B. Mehlig, Bernhard Goksör, Mattias Entrainment of heterogeneous glycolytic oscillations in single cells |
title | Entrainment of heterogeneous glycolytic oscillations in single cells |
title_full | Entrainment of heterogeneous glycolytic oscillations in single cells |
title_fullStr | Entrainment of heterogeneous glycolytic oscillations in single cells |
title_full_unstemmed | Entrainment of heterogeneous glycolytic oscillations in single cells |
title_short | Entrainment of heterogeneous glycolytic oscillations in single cells |
title_sort | entrainment of heterogeneous glycolytic oscillations in single cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371117/ https://www.ncbi.nlm.nih.gov/pubmed/25802053 http://dx.doi.org/10.1038/srep09404 |
work_keys_str_mv | AT gustavssonannakarin entrainmentofheterogeneousglycolyticoscillationsinsinglecells AT adielscarolineb entrainmentofheterogeneousglycolyticoscillationsinsinglecells AT mehligbernhard entrainmentofheterogeneousglycolyticoscillationsinsinglecells AT goksormattias entrainmentofheterogeneousglycolyticoscillationsinsinglecells |