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Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection
Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive inst...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371189/ https://www.ncbi.nlm.nih.gov/pubmed/25801042 http://dx.doi.org/10.1038/srep09152 |
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author | Tokel, Onur Yildiz, Umit Hakan Inci, Fatih Durmus, Naside Gozde Ekiz, Okan Oner Turker, Burak Cetin, Can Rao, Shruthi Sridhar, Kaushik Natarajan, Nalini Shafiee, Hadi Dana, Aykutlu Demirci, Utkan |
author_facet | Tokel, Onur Yildiz, Umit Hakan Inci, Fatih Durmus, Naside Gozde Ekiz, Okan Oner Turker, Burak Cetin, Can Rao, Shruthi Sridhar, Kaushik Natarajan, Nalini Shafiee, Hadi Dana, Aykutlu Demirci, Utkan |
author_sort | Tokel, Onur |
collection | PubMed |
description | Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid, and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e., Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ~10(5) to 3.2 × 10(7) CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings. |
format | Online Article Text |
id | pubmed-4371189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43711892015-04-06 Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection Tokel, Onur Yildiz, Umit Hakan Inci, Fatih Durmus, Naside Gozde Ekiz, Okan Oner Turker, Burak Cetin, Can Rao, Shruthi Sridhar, Kaushik Natarajan, Nalini Shafiee, Hadi Dana, Aykutlu Demirci, Utkan Sci Rep Article Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid, and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e., Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ~10(5) to 3.2 × 10(7) CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings. Nature Publishing Group 2015-03-24 /pmc/articles/PMC4371189/ /pubmed/25801042 http://dx.doi.org/10.1038/srep09152 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Tokel, Onur Yildiz, Umit Hakan Inci, Fatih Durmus, Naside Gozde Ekiz, Okan Oner Turker, Burak Cetin, Can Rao, Shruthi Sridhar, Kaushik Natarajan, Nalini Shafiee, Hadi Dana, Aykutlu Demirci, Utkan Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection |
title | Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection |
title_full | Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection |
title_fullStr | Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection |
title_full_unstemmed | Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection |
title_short | Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection |
title_sort | portable microfluidic integrated plasmonic platform for pathogen detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371189/ https://www.ncbi.nlm.nih.gov/pubmed/25801042 http://dx.doi.org/10.1038/srep09152 |
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