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Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease

Trypanosoma cruzi strains from distinct geographic areas show differences in drug resistance and association between parasites genetic and treatment response has been observed. Considering that benznidazole (BZ) can reduce the parasite burden and tissues damage, even in not cured animals and individ...

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Autores principales: de Oliveira-Silva, Jaquelline Carla Valamiel, Machado-de-Assis, Girley Francisco, Oliveira, Maykon Tavares, Paiva, Nívia Carolina Noguieira, Araújo, Márcio Sobreira Silva, Carneiro, Cláudia Martins, Martins-Filho, Olindo Assis, Martins, Helen Rodrigues, de Lana, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371221/
https://www.ncbi.nlm.nih.gov/pubmed/25742267
http://dx.doi.org/10.1590/0074-02760140260
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author de Oliveira-Silva, Jaquelline Carla Valamiel
Machado-de-Assis, Girley Francisco
Oliveira, Maykon Tavares
Paiva, Nívia Carolina Noguieira
Araújo, Márcio Sobreira Silva
Carneiro, Cláudia Martins
Martins-Filho, Olindo Assis
Martins, Helen Rodrigues
de Lana, Marta
author_facet de Oliveira-Silva, Jaquelline Carla Valamiel
Machado-de-Assis, Girley Francisco
Oliveira, Maykon Tavares
Paiva, Nívia Carolina Noguieira
Araújo, Márcio Sobreira Silva
Carneiro, Cláudia Martins
Martins-Filho, Olindo Assis
Martins, Helen Rodrigues
de Lana, Marta
author_sort de Oliveira-Silva, Jaquelline Carla Valamiel
collection PubMed
description Trypanosoma cruzi strains from distinct geographic areas show differences in drug resistance and association between parasites genetic and treatment response has been observed. Considering that benznidazole (BZ) can reduce the parasite burden and tissues damage, even in not cured animals and individuals, the goal is to assess the drug response to BZ of T. cruzi II strains isolated from children of the Jequitinhonha Valley, state of Minas Gerais, Brazil, before treatment. Mice infected and treated with BZ in both phases of infection were compared with the untreated and evaluated by fresh blood examination, haemoculture, polymerase chain reaction, conventional (ELISA) and non-conventional (FC-ALTA) serologies. In mice treated in the acute phase, a significant decrease in parasitaemia was observed for all strains. Positive parasitological and/or serological tests in animals treated during the acute and chronic (95.1-100%) phases showed that most of the strains were BZ resistant. However, beneficial effect was demonstrated because significant reduction (p < 0.05%) and/or suppression of parasitaemia was observed in mice infected with all strains (acute phase), associated to reduction/elimination of inflammation and fibrosis for two/eight strains. BZ offered some benefit, even in not cured animals, what suggest that BZ use may be recommended at least for recent chronic infection of the studied region.
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spelling pubmed-43712212015-03-25 Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease de Oliveira-Silva, Jaquelline Carla Valamiel Machado-de-Assis, Girley Francisco Oliveira, Maykon Tavares Paiva, Nívia Carolina Noguieira Araújo, Márcio Sobreira Silva Carneiro, Cláudia Martins Martins-Filho, Olindo Assis Martins, Helen Rodrigues de Lana, Marta Mem Inst Oswaldo Cruz Articles Trypanosoma cruzi strains from distinct geographic areas show differences in drug resistance and association between parasites genetic and treatment response has been observed. Considering that benznidazole (BZ) can reduce the parasite burden and tissues damage, even in not cured animals and individuals, the goal is to assess the drug response to BZ of T. cruzi II strains isolated from children of the Jequitinhonha Valley, state of Minas Gerais, Brazil, before treatment. Mice infected and treated with BZ in both phases of infection were compared with the untreated and evaluated by fresh blood examination, haemoculture, polymerase chain reaction, conventional (ELISA) and non-conventional (FC-ALTA) serologies. In mice treated in the acute phase, a significant decrease in parasitaemia was observed for all strains. Positive parasitological and/or serological tests in animals treated during the acute and chronic (95.1-100%) phases showed that most of the strains were BZ resistant. However, beneficial effect was demonstrated because significant reduction (p < 0.05%) and/or suppression of parasitaemia was observed in mice infected with all strains (acute phase), associated to reduction/elimination of inflammation and fibrosis for two/eight strains. BZ offered some benefit, even in not cured animals, what suggest that BZ use may be recommended at least for recent chronic infection of the studied region. Instituto Oswaldo Cruz, Ministério da Saúde 2015-02 /pmc/articles/PMC4371221/ /pubmed/25742267 http://dx.doi.org/10.1590/0074-02760140260 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
de Oliveira-Silva, Jaquelline Carla Valamiel
Machado-de-Assis, Girley Francisco
Oliveira, Maykon Tavares
Paiva, Nívia Carolina Noguieira
Araújo, Márcio Sobreira Silva
Carneiro, Cláudia Martins
Martins-Filho, Olindo Assis
Martins, Helen Rodrigues
de Lana, Marta
Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease
title Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease
title_full Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease
title_fullStr Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease
title_full_unstemmed Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease
title_short Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease
title_sort experimental benznidazole treatment of trypanosoma cruzi ii strains isolated from children of the jequitinhonha valley, minas gerais, brazil, with chagas disease
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371221/
https://www.ncbi.nlm.nih.gov/pubmed/25742267
http://dx.doi.org/10.1590/0074-02760140260
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