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Freshly frozen E18 rat cortical cells can generate functional neural networks after standard cryopreservation and thawing procedures
Primary dissociated brain tissue from rodents is widely used in a variety of different scientific methods to investigate cellular processes in vitro. Often, for this purpose cell cultures need to be generated just on time, requiring extensive animal lab infrastructure. We show here that cryopreserva...
Autores principales: | Quasthoff, Kim, Ferrea, Stefano, Fleischer, Wiebke, Theiss, Stephan, Schnitzler, Alfons, Dihné, Marcel, Walter, Janine |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371571/ https://www.ncbi.nlm.nih.gov/pubmed/24563206 http://dx.doi.org/10.1007/s10616-014-9700-9 |
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