Cargando…

Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae

Filamentous hyphae of the human pathogen, Candida albicans, invade mucosal layers and medical silicones. In vitro, hyphal tips reorient thigmotropically on contact with small obstacles. It is not known how surface topography is sensed but hyphae lacking the cortical marker, Rsr1/Bud1, are unresponsi...

Descripción completa

Detalles Bibliográficos
Autores principales: Thomson, Darren D, Wehmeier, Silvia, Byfield, FitzRoy J, Janmey, Paul A, Caballero-Lima, David, Crossley, Alison, Brand, Alexandra C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371639/
https://www.ncbi.nlm.nih.gov/pubmed/25262778
http://dx.doi.org/10.1111/cmi.12369
_version_ 1782363071175458816
author Thomson, Darren D
Wehmeier, Silvia
Byfield, FitzRoy J
Janmey, Paul A
Caballero-Lima, David
Crossley, Alison
Brand, Alexandra C
author_facet Thomson, Darren D
Wehmeier, Silvia
Byfield, FitzRoy J
Janmey, Paul A
Caballero-Lima, David
Crossley, Alison
Brand, Alexandra C
author_sort Thomson, Darren D
collection PubMed
description Filamentous hyphae of the human pathogen, Candida albicans, invade mucosal layers and medical silicones. In vitro, hyphal tips reorient thigmotropically on contact with small obstacles. It is not known how surface topography is sensed but hyphae lacking the cortical marker, Rsr1/Bud1, are unresponsive. We show that, on surfaces, the morphology of hyphal tips and the position of internal polarity protein complexes are asymmetrically skewed towards the substratum and biased towards the softer of two surfaces. In nano-fabricated chambers, the Spitzenkörper (Spk) responded to touch by translocating across the apex towards the point of contact, where its stable maintenance correlated with contour-following growth. In the rsr1Δ mutant, the position of the Spk meandered and these responses were attenuated. Perpendicular collision caused lateral Spk oscillation within the tip until after establishment of a new growth axis, suggesting Spk position does not predict the direction of growth in C. albicans. Acute tip reorientation occurred only in cells where forward growth was countered by hyphal friction sufficient to generate a tip force of ∼ 8.7 μN (1.2 MPa), more than that required to penetrate host cell membranes. These findings suggest mechanisms through which the organization of hyphal tip growth in C. albicans facilitates the probing, penetration and invasion of host tissue.
format Online
Article
Text
id pubmed-4371639
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BlackWell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-43716392015-03-26 Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae Thomson, Darren D Wehmeier, Silvia Byfield, FitzRoy J Janmey, Paul A Caballero-Lima, David Crossley, Alison Brand, Alexandra C Cell Microbiol Original Articles Filamentous hyphae of the human pathogen, Candida albicans, invade mucosal layers and medical silicones. In vitro, hyphal tips reorient thigmotropically on contact with small obstacles. It is not known how surface topography is sensed but hyphae lacking the cortical marker, Rsr1/Bud1, are unresponsive. We show that, on surfaces, the morphology of hyphal tips and the position of internal polarity protein complexes are asymmetrically skewed towards the substratum and biased towards the softer of two surfaces. In nano-fabricated chambers, the Spitzenkörper (Spk) responded to touch by translocating across the apex towards the point of contact, where its stable maintenance correlated with contour-following growth. In the rsr1Δ mutant, the position of the Spk meandered and these responses were attenuated. Perpendicular collision caused lateral Spk oscillation within the tip until after establishment of a new growth axis, suggesting Spk position does not predict the direction of growth in C. albicans. Acute tip reorientation occurred only in cells where forward growth was countered by hyphal friction sufficient to generate a tip force of ∼ 8.7 μN (1.2 MPa), more than that required to penetrate host cell membranes. These findings suggest mechanisms through which the organization of hyphal tip growth in C. albicans facilitates the probing, penetration and invasion of host tissue. BlackWell Publishing Ltd 2015-03 2014-11-25 /pmc/articles/PMC4371639/ /pubmed/25262778 http://dx.doi.org/10.1111/cmi.12369 Text en © 2014 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Thomson, Darren D
Wehmeier, Silvia
Byfield, FitzRoy J
Janmey, Paul A
Caballero-Lima, David
Crossley, Alison
Brand, Alexandra C
Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae
title Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae
title_full Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae
title_fullStr Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae
title_full_unstemmed Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae
title_short Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae
title_sort contact-induced apical asymmetry drives the thigmotropic responses of candida albicans hyphae
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371639/
https://www.ncbi.nlm.nih.gov/pubmed/25262778
http://dx.doi.org/10.1111/cmi.12369
work_keys_str_mv AT thomsondarrend contactinducedapicalasymmetrydrivesthethigmotropicresponsesofcandidaalbicanshyphae
AT wehmeiersilvia contactinducedapicalasymmetrydrivesthethigmotropicresponsesofcandidaalbicanshyphae
AT byfieldfitzroyj contactinducedapicalasymmetrydrivesthethigmotropicresponsesofcandidaalbicanshyphae
AT janmeypaula contactinducedapicalasymmetrydrivesthethigmotropicresponsesofcandidaalbicanshyphae
AT caballerolimadavid contactinducedapicalasymmetrydrivesthethigmotropicresponsesofcandidaalbicanshyphae
AT crossleyalison contactinducedapicalasymmetrydrivesthethigmotropicresponsesofcandidaalbicanshyphae
AT brandalexandrac contactinducedapicalasymmetrydrivesthethigmotropicresponsesofcandidaalbicanshyphae